Overview
| Uniprot ID | Q96CF2 |
|---|
| Protein Name | Charged multivesicular body protein 4c |
|---|
| Gene Name | CHMP4C |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 9 |
SKLGKFFKGGGSSKS |
Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission (PubMed:22422861, PubMed:24814515). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413)
Protein Sequence
10
MSKLGKFFKG
20
GGSSKSRAAP
30
SPQEALVRLR
40
ETEEMLGKKQ
50
EYLENRIQRE
60
IALAKKHGTQ
70
NKRAALQALK
80
RKKRFEKQLT
90
QIDGTLSTIE
100
FQREALENSH
110
TNTEVLRNMG
120
FAAKAMKSVH
130
ENMDLNKIDD
140
LMQEITEQQD
150
IAQEISEAFS
160
QRVGFGDDFD
170
EDELMAELEE
180
LEQEELNKKM
190
TNIRLPNVPS
200
SSLPAQPNRK
210
PGMSSTARRS
220
RAASSQRAEE
230
EDDDIKQLAA
WAT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:1904930 |
amphisome membrane |
| Cellular Component |
GO:0000421 |
autophagosome membrane |
| Cellular Component |
GO:0009898 |
cytoplasmic side of plasma membrane |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0000815 |
ESCRT III complex |
| Cellular Component |
GO:0090543 |
Flemming body |
| Cellular Component |
GO:0000776 |
kinetochore |
| Cellular Component |
GO:0005828 |
kinetochore microtubule |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0005771 |
multivesicular body |
| Cellular Component |
GO:0032585 |
multivesicular body membrane |
| Cellular Component |
GO:0005635 |
nuclear envelope |
| Cellular Component |
GO:0005643 |
nuclear pore |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Biological Process |
GO:0097352 |
autophagosome maturation |
| Biological Process |
GO:0006914 |
autophagy |
| Biological Process |
GO:1902774 |
late endosome to lysosome transport |
| Biological Process |
GO:0032511 |
late endosome to vacuole transport via multivesicular body sorting pathway |
| Biological Process |
GO:0016236 |
macroautophagy |
| Biological Process |
GO:0090148 |
membrane fission |
| Biological Process |
GO:0061952 |
midbody abscission |
| Biological Process |
GO:0044878 |
mitotic cytokinesis checkpoint signaling |
| Biological Process |
GO:0007080 |
mitotic metaphase chromosome alignment |
| Biological Process |
GO:0036258 |
multivesicular body assembly |
| Biological Process |
GO:0071985 |
multivesicular body sorting pathway |
| Biological Process |
GO:0061763 |
multivesicular body-lysosome fusion |
| Biological Process |
GO:0032466 |
negative regulation of cytokinesis |
| Biological Process |
GO:0031468 |
nuclear membrane reassembly |
| Biological Process |
GO:0006997 |
nucleus organization |
| Biological Process |
GO:0001778 |
plasma membrane repair |
| Biological Process |
GO:0015031 |
protein transport |
| Biological Process |
GO:0010824 |
regulation of centrosome duplication |
| Biological Process |
GO:1901673 |
regulation of mitotic spindle assembly |
| Biological Process |
GO:0043162 |
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
| Biological Process |
GO:0090611 |
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway |
| Biological Process |
GO:0006900 |
vesicle budding from membrane |
| Biological Process |
GO:0051469 |
vesicle fusion with vacuole |
| Biological Process |
GO:0046761 |
viral budding from plasma membrane |
| Biological Process |
GO:0039702 |
viral budding via host ESCRT complex |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.