Overview
| Uniprot ID | Q96CS3 |
|---|
| Protein Name | FAS-associated factor 2 |
|---|
| Gene Name | FAF2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 329 |
EEEVQQQKLAEERRR |
Function
Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:18711132, PubMed:24215460). By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed:26692333). Recruits ATPase VCP to lipid droplets and also binds to phospholipase PNPLA2/ATGL, inhibiting PNPLA2 activity by promoting its dissociation from its ABHD5/CGI-58 activator (PubMed:23297223). This inhibits PNPLA2-mediated triacylglycerol hydrolysis and leads to inhibition of lipid droplet degradation (PubMed:23297223). Involved in stress granule disassembly: associates with ubiquitinated G3BP1 in response to heat shock, thereby promoting interaction between ubiquitinated G3BP1 and VCP, followed by G3BP1 extraction from stress granules and stress granule disassembly (PubMed:34739333)
Protein Sequence
10
MAAPEERDLT
20
QEQTEKLLQF
30
QDLTGIESMD
40
QCRHTLEQHN
50
WNIEAAVQDR
60
LNEQEGVPSV
70
FNPPPSRPLQ
80
VNTADHRIYS
90
YVVSRPQPRG
100
LLGWGYYLIM
110
LPFRFTYYTI
120
LDIFRFALRF
130
IRPDPRSRVT
140
DPVGDIVSFM
150
HSFEEKYGRA
160
HPVFYQGTYS
170
QALNDAKREL
180
RFLLVYLHGD
190
DHQDSDEFCR
200
NTLCAPEVIS
210
LINTRMLFWA
220
CSTNKPEGYR
230
VSQALRENTY
240
PFLAMIMLKD
250
RRMTVVGRLE
260
GLIQPDDLIN
270
QLTFIMDANQ
280
TYLVSERLER
290
EERNQTQVLR
300
QQQDEAYLAS
310
LRADQEKERK
320
KREERERKRR
330
KEEEVQQQKL
340
AEERRRQNLQ
350
EEKERKLECL
360
PPEPSPDDPE
370
SVKIIFKLPN
380
DSRVERRFHF
390
SQSLTVIHDF
400
LFSLKESPEK
410
FQIEANFPRR
420
VLPCIPSEEW
430
PNPPTLQEAG
440
LSHTEVLFVQ
DLTDE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035578 |
azurophil granule lumen |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005576 |
extracellular region |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Cellular Component |
GO:0034098 |
VCP-NPL4-UFD1 AAA ATPase complex |
| Molecular Function |
GO:0035473 |
lipase binding |
| Molecular Function |
GO:0055102 |
lipase inhibitor activity |
| Molecular Function |
GO:0030674 |
protein-macromolecule adaptor activity |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Biological Process |
GO:0036503 |
ERAD pathway |
| Biological Process |
GO:0034389 |
lipid droplet organization |
| Biological Process |
GO:0043161 |
proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0006986 |
response to unfolded protein |
| Biological Process |
GO:0030970 |
retrograde protein transport, ER to cytosol |
| Biological Process |
GO:0035617 |
stress granule disassembly |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.