Overview
| Uniprot ID | Q96EA4 |
|---|
| Protein Name | Protein Spindly |
|---|
| Gene Name | SPDL1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 450 |
TVEVPVLKKRREVLP |
| 451 |
VEVPVLKKRREVLPV |
| 534 |
KKEKKCVKLIGVPAD |
| 563 |
PRLAAESKLQTEVKE |
Function
Required for the localization of dynein and dynactin to the mitotic kinetochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494). Plays a role in cell migration (PubMed:30258100)
Protein Sequence
10
MEADIITNLR
20
CRLKEAEEER
30
LKAAQYGLQL
40
VESQNELQNQ
50
LDKCRNEMMT
60
MTESYEQEKY
70
TLQREVELKS
80
RMLESLSCEC
90
EAIKQQQKMH
100
LEKLEEQLSR
110
SHGQEVNELK
120
TKIEKLKVEL
130
DEARLSEKQL
140
KHQVDHQKEL
150
LSCKSEELRV
160
MSERVQESMS
170
SEMLALQIEL
180
TEMESMKTTL
190
KEEVNELQYR
200
QEQLELLITN
210
LMRQVDRLKE
220
EKEEREKEAV
230
SYYNALEKAR
240
VANQDLQVQL
250
DQALQQALDP
260
NSKGNSLFAE
270
VEDRRAAMER
280
QLISMKVKYQ
290
SLKKQNVFNR
300
EQMQRMKLQI
310
ATLLQMKGSQ
320
TEFEQQERLL
330
AMLEQKNGEI
340
KHLLGEIRNL
350
EKFKNLYDSM
360
ESKPSVDSGT
370
LEDNTYYTDL
380
LQMKLDNLNK
390
EIESTKGELS
400
IQRMKALFES
410
QRALDIERKL
420
FANERCLQLS
430
ESENMKLRAK
440
LDELKLKYEP
450
EETVEVPVLK
460
KRREVLPVDI
470
TTAKDACVNN
480
SALGGEVYRL
490
PPQKEETQSC
500
PNSLEDNNLQ
510
LEKSVSIYTP
520
VVSLSPHKNL
530
PVDMQLKKEK
540
KCVKLIGVPA
550
DAEALSERSG
560
NTPNSPRLAA
570
ESKLQTEVKE
580
GKETSSKLEK
590
ETCKKLHPIL
600
YVSSKSTPET
QCPQQ
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0000940 |
outer kinetochore |
| Cellular Component |
GO:0000922 |
spindle pole |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0043515 |
kinetochore binding |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0016477 |
cell migration |
| Biological Process |
GO:0000132 |
establishment of mitotic spindle orientation |
| Biological Process |
GO:0007080 |
mitotic metaphase chromosome alignment |
| Biological Process |
GO:0007094 |
mitotic spindle assembly checkpoint signaling |
| Biological Process |
GO:0034501 |
protein localization to kinetochore |
Reference
[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.