Overview
| Uniprot ID | Q96EP0 |
|---|
| Protein Name | E3 ubiquitin-protein ligase RNF31 |
|---|
| Gene Name | RNF31 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 988 |
LRDEACGKETPAGYA |
Function
E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:17006537, PubMed:19136968, PubMed:20005846, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22863777, PubMed:28189684, PubMed:28481331). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed:17006537, PubMed:19136968, PubMed:20005846, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22863777, PubMed:28189684). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:21455173, PubMed:28189684). LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed:20005846, PubMed:27458237). The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed:28481331, PubMed:34012115). LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed:28481331). Recruited to the surface of bacteria by RNF213, which initiates the bacterial ubiquitin coat (PubMed:34012115). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed:28481331, PubMed:34012115). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed:23708998). RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation (PubMed:27777308). Binds polyubiquitin of different linkage types (PubMed:23708998)
Protein Sequence
10
MPGEEEERAF
20
LVAREELASA
30
LRRDSGQAFS
40
LEQLRPLLAS
50
SLPLAARYLQ
60
LDAARLVRCN
70
AHGEPRNYLN
80
TLSTALNILE
90
KYGRNLLSPQ
100
RPRYWRGVKF
110
NNPVFRSTVD
120
AVQGGRDVLR
130
LYGYTEEQPD
140
GLSFPEGQEE
150
PDEHQVATVT
160
LEVLLLRTEL
170
SLLLQNTHPR
180
QQALEQLLED
190
KVEDDMLQLS
200
EFDPLLREIA
210
PGPLTTPSVP
220
GSTPGPCFLC
230
GSAPGTLHCP
240
SCKQALCPAC
250
DHLFHGHPSR
260
AHHLRQTLPG
270
VLQGTHLSPS
280
LPASAQPRPQ
290
STSLLALGDS
300
SLSSPNPASA
310
HLPWHCAACA
320
MLNEPWAVLC
330
VACDRPRGCK
340
GLGLGTEGPQ
350
GTGGLEPDLA
360
RGRWACQSCT
370
FENEAAAVLC
380
SICERPRLAQ
390
PPSLVVDSRD
400
AGICLQPLQQ
410
GDALLASAQS
420
QVWYCIHCTF
430
CNSSPGWVCV
440
MCNRTSSPIP
450
AQHAPRPYAS
460
SLEKGPPKPG
470
PPRRLSAPLP
480
SSCGDPEKQR
490
QDKMREEGLQ
500
LVSMIREGEA
510
AGACPEEIFS
520
ALQYSGTEVP
530
LQWLRSELPY
540
VLEMVAELAG
550
QQDPGLGAFS
560
CQEARRAWLD
570
RHGNLDEAVE
580
ECVRTRRRKV
590
QELQSLGFGP
600
EEGSLQALFQ
610
HGGDVSRALT
620
ELQRQRLEPF
630
RQRLWDSGPE
640
PTPSWDGPDK
650
QSLVRRLLAV
660
YALPSWGRAE
670
LALSLLQETP
680
RNYELGDVVE
690
AVRHSQDRAF
700
LRRLLAQECA
710
VCGWALPHNR
720
MQALTSCECT
730
ICPDCFRQHF
740
TIALKEKHIT
750
DMVCPACGRP
760
DLTDDTQLLS
770
YFSTLDIQLR
780
ESLEPDAYAL
790
FHKKLTEGVL
800
MRDPKFLWCA
810
QCSFGFIYER
820
EQLEATCPQC
830
HQTFCVRCKR
840
QWEEQHRGRS
850
CEDFQNWKRM
860
NDPEYQAQGL
870
AMYLQENGID
880
CPKCKFSYAL
890
ARGGCMHFHC
900
TQCRHQFCSG
910
CYNAFYAKNK
920
CPEPNCRVKK
930
SLHGHHPRDC
940
LFYLRDWTAL
950
RLQKLLQDNN
960
VMFNTEPPAG
970
ARAVPGGGCR
980
VIEQKEVPNG
990
LRDEACGKET
1000
PAGYAGLCQA
1010
HYKEYLVSLI
1020
NAHSLDPATL
1030
YEVEELETAT
1040
ERYLHVRPQP
1050
LAGEDPPAYQ
1060
ARLLQKLTEE
1070
VPLGQSIPRR
RK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035631 |
CD40 receptor complex |
| Cellular Component |
GO:0009898 |
cytoplasmic side of plasma membrane |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0071797 |
LUBAC complex |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0036435 |
K48-linked polyubiquitin modification-dependent protein binding |
| Molecular Function |
GO:0070530 |
K63-linked polyubiquitin modification-dependent protein binding |
| Molecular Function |
GO:1990450 |
linear polyubiquitin binding |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Molecular Function |
GO:0004842 |
ubiquitin-protein transferase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0023035 |
CD40 signaling pathway |
| Biological Process |
GO:0042742 |
defense response to bacterium |
| Biological Process |
GO:0043123 |
positive regulation of canonical NF-kappaB signal transduction |
| Biological Process |
GO:1903955 |
positive regulation of protein targeting to mitochondrion |
| Biological Process |
GO:1904417 |
positive regulation of xenophagy |
| Biological Process |
GO:0097039 |
protein linear polyubiquitination |
| Biological Process |
GO:0000209 |
protein polyubiquitination |
| Biological Process |
GO:0050852 |
T cell receptor signaling pathway |
Reference
PMID: N/A