Search Results

Overview

Uniprot IDQ96EV2
Protein NameRNA-binding protein 33
Gene NameRBM33
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1061 IQGIHPAKKAIMHGR
1062 QGIHPAKKAIMHGRG
1085 RGRLMPNKQNLRVVE
692 TTSQNVSKRPMQQMQ
798 RLKIEEQKRLREEIL
877 VKNRLLVKNQDVSIS
908 QYQGQQMKALKHLRQ
929 SQTQPLHKVLPIKPA
984 DGPHISSKVRVIKLS
989 SSKVRVIKLSGGGGE

Function

RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as mRNA export, mRNA stability and/or translation (PubMed:35589130, PubMed:37257451). Binds a subset of intronless RNAs containing GC-rich elements, such as NORAD, and promotes their nuclear export by recruiting target RNAs to components of the NXF1-NXT1 RNA export machinery (PubMed:35589130). Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, promoting their demethylation by ALKBH5 (PubMed:37257451). Acts as an molecular adapter, which (1) promotes ALKBH5 recruitment to m6A-containing transcripts and (2) activates ALKBH5 demethylase activity by recruiting SENP1, leading to ALKBH5 deSUMOylation and subsequent activation (PubMed:37257451)

Protein Sequence

10 MAAALGASGG 20 AGAGDDDFDQ 30 FDKPGAERSW 40 RRRAADEDWD 50 SELEDDLLGE 60 DLLSGKKNQS 70 DLSDEELNDD 80 LLQSDNEDEE 90 NFSSQGVTIS 100 LNATSGMVTS 110 FELSDNTNDQ 120 SGEQESEYEQ 130 EQGEDELVYH 140 KSDGSELYTQ 150 EYPEEGQYEG 160 HEAELTEDQI 170 EYVEEPEEEQ 180 LYTDEVLDIE 190 INEPLDEFTG 200 GMETLELQKD 210 IKEESDEEEE 220 DDEESGRLRF 230 KTERKEGTII 240 RLSDVTRERR 250 NIPETLELSA 260 EAKAALLEFE 270 ERERQHKQGR 280 YSSRRGGRRG 290 GPLMCRGVGD 300 QRRESTERGR 310 MKDHRPALLP 320 TQPPVVPQAP 330 PPPPPPPQQQ 340 PIRSLFQPQP 350 LQPLLPVQHP 360 HHPSPPQGMH 370 MPPQLETPRM 380 MMTPPPVTPQ 390 QPKNIHINPH 400 FKGTVVTPVQ 410 VPLLPVPSQP 420 RPAVGPQRFP 430 GPPEFPQHTP 440 GPVPNSFSQP 450 PRLPLQDQWR 460 APPPPQDRDP 470 FFLGVSGEPR 480 FPSHLFLEQR 490 SPPPPPPPPT 500 LLNSSHPVPT 510 QSPLPFTQPG 520 PAFNQQGQQP 530 VFPRERPVRP 540 ALQPPGPVGI 550 LHFSQPGSAT 560 TRPFIPPRQP 570 FLPGPGQPFL 580 PTHTQPNLQG 590 PLHPPLPPPH 600 QPQPQQPQQQ 610 PPPQHQPPHQ 620 PPHQPPPQHQ 630 PPPQHPPQHP 640 PQHQHHHHHH 650 HLSVPPPPLM 660 PMSQPQFRPH 670 VQTAQPQASS 680 SRMQCPQRQG 690 LRHNTTSQNV 700 SKRPMQQMQP 710 TAPRNSNLRE 720 LPIAPSHVIE 730 MSSSRCSATP 740 SAQVKPIVSA 750 SPPSRAVAGS 760 RSSQGKTEVK 770 VKPASPVAQP 780 KEEAKTETEF 790 PDEDEETRLY 800 RLKIEEQKRL 810 REEILKQKEL 820 RRQQQAGARK 830 KELLERLAQQ 840 QQQLYAPPPP 850 AEQEEQALSP 860 SPTNGNPLLP 870 FPGAQVRQNV 880 KNRLLVKNQD 890 VSISNVQPKT 900 SNFVPSSANM 910 QYQGQQMKAL 920 KHLRQTRTVP 930 QSQTQPLHKV 940 LPIKPADVEE 950 PAVPQTPRVA 960 SIQGRPQDTK 970 PGVKRTVTHR 980 TNSGGGDGPH 990 ISSKVRVIKL 1000 SGGGGESDGF 1010 FHPEGQPQRL 1020 PQPPEVGPQP 1030 ARKVTLTRGG 1040 LQQPPHLPAG 1050 PHAHSPVPPG 1060 IKSIQGIHPA 1070 KKAIMHGRGR 1080 GVAGPMGRGR 1090 LMPNKQNLRV 1100 VECKPQPCVV 1110 SVEGLSSSTT 1120 DAQLKSLLMS 1130 VGPIQSLQML 1140 PQQRKAIAKF 1150 KEPAHALAFQ 1160 QKFHRHMIDL 1170 SHINVALIVE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0106222 lncRNA binding
Molecular Function GO:1990247 N6-methyladenosine-containing RNA reader activity
Molecular Function GO:0030674 protein-macromolecule adaptor activity
Molecular Function GO:0003723 RNA binding
Biological Process GO:0051028 mRNA transport
Biological Process GO:0043488 regulation of mRNA stability
Biological Process GO:0006405 RNA export from nucleus

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.