Search Results

Overview

Uniprot IDQ96I25
Protein NameSplicing factor 45
Gene NameRBM17
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
206 RPRSQSSKAAIPPPV
21 SKTEGWSKNFKLLQS
24 EGWSKNFKLLQSQLQ
245 VAHKIMQKYGFREGQ
256 REGQGLGKHEQGLST
269 STALSVEKTSKRGGK
276 KTSKRGGKIIVGDAT
285 IVGDATEKDASKKSD
290 TEKDASKKSDSNPLT
33 LQSQLQVKKAALTQA
34 QSQLQVKKAALTQAK
41 KAALTQAKSQRTKQS
46 QAKSQRTKQSTVLAP
58 LAPVIDLKRGGSSDD

Function

Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia

Protein Sequence

10 MSLYDDLGVE 20 TSDSKTEGWS 30 KNFKLLQSQL 40 QVKKAALTQA 50 KSQRTKQSTV 60 LAPVIDLKRG 70 GSSDDRQIVD 80 TPPHVAAGLK 90 DPVPSGFSAG 100 EVLIPLADEY 110 DPMFPNDYEK 120 VVKRQREERQ 130 RQRELERQKE 140 IEEREKRRKD 150 RHEASGFARR 160 PDPDSDEDED 170 YERERRKRSM 180 GGAAIAPPTS 190 LVEKDKELPR 200 DFPYEEDSRP 210 RSQSSKAAIP 220 PPVYEEQDRP 230 RSPTGPSNSF 240 LANMGGTVAH 250 KIMQKYGFRE 260 GQGLGKHEQG 270 LSTALSVEKT 280 SKRGGKIIVG 290 DATEKDASKK 300 SDSNPLTEIL 310 KCPTKVVLLR 320 NMVGAGEVDE 330 DLEVETKEEC 340 EKYGKVGKCV 350 IFEIPGAPDD 360 EAVRIFLEFE 370 RVESAIKAVV 380 DLNGRYFGGR 390 VVKACFYNLD 400 KFRVLDLAEQ V

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005681 spliceosomal complex
Molecular Function GO:0003723 RNA binding
Biological Process GO:0000380 alternative mRNA splicing, via spliceosome
Biological Process GO:0045292 mRNA cis splicing, via spliceosome

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[6] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[7] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[8] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[9] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[10] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[11] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.