Search Results

Overview

Uniprot IDQ96I99
Protein NameSuccinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
Gene NameSUCLG2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
101 GVFNSGLKGGVHLTK
108 KGGVHLTKDPNVVGQ
118 NVVGQLAKQMIGYNL
128 IGYNLATKQTPKEGV
132 LATKQTPKEGVKVNK
139 KEGVKVNKVMVAEAL
200 IDIFEGIKDSQAQRM
291 PIENEAAKYDLKYIG
47 LQEYQSKKLMSDNGV
73 NEALEAAKRLNAKEI
78 AAKRLNAKEIVLKAQ
83 NAKEIVLKAQILAGG
93 ILAGGRGKGVFNSGL

Function

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed:40108300). The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit (By similarity). Also able to act as a GTP-specific itaconyl- and malyl-CoA synthetase (PubMed:40108300)

Protein Sequence

10 MASPVAAQAG 20 KLLRALALRP 30 RFLAAGSQAV 40 QLTSRRWLNL 50 QEYQSKKLMS 60 DNGVRVQRFF 70 VADTANEALE 80 AAKRLNAKEI 90 VLKAQILAGG 100 RGKGVFNSGL 110 KGGVHLTKDP 120 NVVGQLAKQM 130 IGYNLATKQT 140 PKEGVKVNKV 150 MVAEALDISR 160 ETYLAILMDR 170 SCNGPVLVGS 180 PQGGVDIEEV 190 AASNPELIFK 200 EQIDIFEGIK 210 DSQAQRMAEN 220 LGFVGPLKSQ 230 AADQITKLYN 240 LFLKIDATQV 250 EVNPFGETPE 260 GQVVCFDAKI 270 NFDDNAEFRQ 280 KDIFAMDDKS 290 ENEPIENEAA 300 KYDLKYIGLD 310 GNIACFVNGA 320 GLAMATCDII 330 FLNGGKPANF 340 LDLGGGVKEA 350 QVYQAFKLLT 360 ADPKVEAILV 370 NIFGGIVNCA 380 IIANGITKAC 390 RELELKVPLV 400 VRLEGTNVQE 410 AQKILNNSGL 420 PITSAIDLED 430 AAKKAVASVA KK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0042709 succinate-CoA ligase complex
Cellular Component GO:0045244 succinate-CoA ligase complex (GDP-forming)
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0019003 GDP binding
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0004776 succinate-CoA ligase (GDP-forming) activity
Biological Process GO:0006105 succinate metabolic process
Biological Process GO:1901289 succinyl-CoA catabolic process
Biological Process GO:0006104 succinyl-CoA metabolic process
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.