Search Results

Overview

Uniprot IDQ96KP4
Protein NameCytosolic non-specific dipeptidase
Gene NameCNDP2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
24 RYIKKLAKWVAIQSV
301 QILLHSHKKDILMHR
302 ILLHSHKKDILMHRW
37 SVSAWPEKRGEIRRM
375 LRSPNEFKVYMGHGG
383 VYMGHGGKPWVSDFS
402 LAGRRAMKTVFGVEP
66 VELVDIGKQKLPDGS

Function

Cytosolic metallodipeptidase with broad substrate specificity that catalyzes the hydrolysis of amide bonds in dipeptides and pseudopeptides (PubMed:12473676, PubMed:19346245, PubMed:25964343). Substrates include L-cysteinylglycine, an intermediate metabolite of the gamma-glutamyl cycle of glutathione metabolism (PubMed:19346245, PubMed:25964343). Can also hydrolyze threonyl-containing dipeptides (By similarity). Upon physiological conditions of amino acids and lactate excess can also catalyze a reverse proteolysis reaction, generating N-[(S)-lactoyl]-L-phenylalanine and possibly other N-lactoyl-amino acids which it can also hydrolyze in vitro. N-[(S)-lactoyl]-L-phenylalanine is an exercise-induced metabolite that influences food intake and systemic energy balance (PubMed:25964343). Carnosine is probably not a physiological substrate and L-homocarnosine is not a substrate for this enzyme (PubMed:12473676). Plays a role in the regulation of cell cycle arrest and apoptosis (PubMed:17121880, PubMed:24395568)

Protein Sequence

10 MAALTTLFKY 20 IDENQDRYIK 30 KLAKWVAIQS 40 VSAWPEKRGE 50 IRRMMEVAAA 60 DVKQLGGSVE 70 LVDIGKQKLP 80 DGSEIPLPPI 90 LLGRLGSDPQ 100 KKTVCIYGHL 110 DVQPAALEDG 120 WDSEPFTLVE 130 RDGKLYGRGS 140 TDDKGPVAGW 150 INALEAYQKT 160 GQEIPVNVRF 170 CLEGMEESGS 180 EGLDELIFAR 190 KDTFFKDVDY 200 VCISDNYWLG 210 KKKPCITYGL 220 RGICYFFIEV 230 ECSNKDLHSG 240 VYGGSVHEAM 250 TDLILLMGSL 260 VDKRGNILIP 270 GINEAVAAVT 280 EEEHKLYDDI 290 DFDIEEFAKD 300 VGAQILLHSH 310 KKDILMHRWR 320 YPSLSLHGIE 330 GAFSGSGAKT 340 VIPRKVVGKF 350 SIRLVPNMTP 360 EVVGEQVTSY 370 LTKKFAELRS 380 PNEFKVYMGH 390 GGKPWVSDFS 400 HPHYLAGRRA 410 MKTVFGVEPD 420 LTREGGSIPV 430 TLTFQEATGK 440 NVMLLPVGSA 450 DDGAHSQNEK 460 LNRYNYIEGT 470 KMLAAYLYEV SQLKD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0004180 carboxypeptidase activity
Molecular Function GO:0016805 dipeptidase activity
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0070573 metallodipeptidase activity
Biological Process GO:0006749 glutathione metabolic process
Biological Process GO:0019184 nonribosomal peptide biosynthetic process
Biological Process GO:0006508 proteolysis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.