Search Results

Overview

Uniprot ID	Q96QE3
Protein Name	ATPase family AAA domain-containing protein 5
Gene Name	ATAD5
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1442	LDNKIYPKNTKKKRV
1445	KIYPKNTKKKRVDLP
189	MTSLQNSKKVNPKQG
41	STCKTITKYLSPLGK
999	SHKETKRKLVEAENS

Function

Has an important role in DNA replication and in maintaining genome integrity during replication stress (PubMed:15983387, PubMed:19755857). Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis (PubMed:15983387). Modulates the RAD9A interaction with BCL2 and thereby induces DNA damage-induced apoptosis (PubMed:15983387). Promotes PCNA deubiquitination by recruiting the ubiquitin-specific protease 1 (USP1) and WDR48 thereby down-regulating the error-prone damage bypass pathway (PubMed:20147293). As component of the ATAD5 RFC-like complex, regulates the function of the DNA polymerase processivity factor PCNA by unloading the ring-shaped PCNA homotrimer from DNA after replication during the S phase of the cell cycle (PubMed:23277426, PubMed:23937667). This seems to be dependent on its ATPase activity (PubMed:23277426). Plays important roles in restarting stalled replication forks under replication stress, by unloading the PCNA homotrimer from DNA and recruiting RAD51 possibly through an ATR-dependent manner (PubMed:31844045). Ultimately this enables replication fork regression, breakage, and eventual fork restart (PubMed:31844045). Both the PCNA unloading activity and the interaction with WDR48 are required to efficiently recruit RAD51 to stalled replication forks (PubMed:31844045). Promotes the generation of MUS81-mediated single-stranded DNA-associated breaks in response to replication stress, which is an alternative pathway to restart stalled/regressed replication forks (PubMed:31844045)

Protein Sequence

10 MVGVLAMAAA 20 AAPPPVKDCE 30 IEPCKKRKKD 40 DDTSTCKTIT 50 KYLSPLGKTR 60 DRVFAPPKPS 70 NILDYFRKTS 80 PTNEKTQLGK 90 ECKIKSPESV 100 PVDSNKDCTT 110 PLEMFSNVEF 120 KKKRKRVNLS 130 HQLNNIKTEN 140 EAPIEISSDD 150 SKEDYSLNND 160 FVESSTSVLR 170 YKKQVEVLAE 180 NIQDTKSQPN 190 TMTSLQNSKK 200 VNPKQGTTKN 210 DFKKLRKRKC 220 RDVVDLSESL 230 PLAEELNLLK 240 KDGKDTKQME 250 NTTSHANSRD 260 NVTEAAQLND 270 SIITVSYEEF 280 LKSHKENKVE 290 EIPDSTMSIC 300 VPSETVDEIV 310 KSGYISESEN 320 SEISQQVRFK 330 TVTVLAQVHP 340 IPPKKTGKIP 350 RIFLKQKQFE 360 MENSLSDPEN 370 EQTVQKRKSN 380 VVIQEEELEL 390 AVLEAGSSEA 400 VKPKCTLEER 410 QQFMKAFRQP 420 ASDALKNGVK 430 KSSDKQKDLN 440 EKCLYEVGRD 450 DNSKKIMENS 460 GIQMVSKNGN 470 LQLHTDKGSF 480 LKEKNKKLKK 490 KNKKTLDTGA 500 IPGKNREGNT 510 QKKETTFFLK 520 EKQYQNRMSL 530 RQRKTEFFKS 540 STLFNNESLV 550 YEDIANDDLL 560 KVSSLCNNNK 570 LSRKTSIPVK 580 DIKLTQSKAE 590 SEASLLNVST 600 PKSTRRSGRI 610 SSTPTTETIR 620 GIDSDDVQDN 630 SQLKASTQKA 640 ANLSEKHSLY 650 TAELITVPFD 660 SESPIRMKFT 670 RISTPKKSKK 680 KSNKRSEKSE 690 ATDGGFTSQI 700 RKASNTSKNI 710 SKAKQLIEKA 720 KALHISRSKV 730 TEEIAIPLRR 740 SSRHQTLPER 750 KKLSETEDSV 760 IIIDSSPTAL 770 KHPEKNQKKL 780 QCLNDVLGKK 790 LNTSTKNVPG 800 KMKVAPLFLV 810 RKAQKAADPV 820 PSFDESSQDT 830 SEKSQDCDVQ 840 CKAKRDFLMS 850 GLPDLLKRQI 860 AKKAAALDVY 870 NAVSTSFQRV 880 VHVQQKDDGC 890 CLWHLKPPSC 900 PLLTKFKELN 910 TKVIDLSKCG 920 IALGEFSTLN 930 SKLKSGNSAA 940 VFMRTRKEFT 950 EEVRNLLLEE 960 IRWSNPEFSL 970 KKYFPLLLKK 980 QIEHQVLSSE 990 CHSKQELEAD 1000 VSHKETKRKL 1010 VEAENSKSKR 1020 KKPNEYSKNL 1030 EKTNRKSEEL 1040 SKRNNSSGIK 1050 LDSSKDSGTE 1060 DMLWTEKYQP 1070 QTASELIGNE 1080 LAIKKLHSWL 1090 KDWKRRAELE 1100 ERQNLKGKRD 1110 EKHEDFSGGI 1120 DFKGSSDDEE 1130 ESRLCNTVLI 1140 TGPTGVGKTA 1150 AVYACAQELG 1160 FKIFEVNASS 1170 QRSGRQILSQ 1180 LKEATQSHQV 1190 DKQGVNSQKP 1200 CFFNSYYIGK 1210 SPKKISSPKK 1220 VVTSPRKVPP 1230 PSPKSSGPKR 1240 ALPPKTLANY 1250 FKVSPKPKNN 1260 EEIGMLLENN 1270 KGIKNSFEQK 1280 QITQTKSTNA 1290 TNSNVKDVGA 1300 EEPSRKNATS 1310 LILFEEVDVI 1320 FDEDAGFLNA 1330 IKTFMATTKR 1340 PVILTTSDPT 1350 FSLMFDGCFE 1360 EIKFSTPSLL 1370 NVASYLQMIC 1380 LTENFRTDVK 1390 DFVTLLTANT 1400 CDIRKSILYL 1410 QFWIRSGGGV 1420 LEERPLTLYR 1430 GNSRNVQLVC 1440 SEHGLDNKIY 1450 PKNTKKKRVD 1460 LPKCDSGCAE 1470 TLFGLKNIFS 1480 PSEDLFSFLK 1490 HKITMKEEWH 1500 KFIQLLTEFQ 1510 MRNVDFLYSN 1520 LEFILPLPVD 1530 TIPETKNFCG 1540 PSVTVDASAA 1550 TKSMNCLARK 1560 HSEREQPLKK 1570 SQKKKQKKTL 1580 VILDDSDLFD 1590 TDLDFPDQSI 1600 SLSSVSSSSN 1610 AEESKTGDEE 1620 SKARDKGNNP 1630 ETKKSIPCPP 1640 KTTAGKKCSA 1650 LVSHCLNSLS 1660 EFMDNMSFLD 1670 ALLTDVREQN 1680 KYGRNDFSWT 1690 NGKVTSGLCD 1700 EFSLESNDGW 1710 TSQSSGELKA 1720 AAEALSFTKC 1730 SSAISKALET 1740 LNSCKKLGRD 1750 PTNDLTFYVS 1760 QKRNNVYFSQ 1770 SAANLDNAWK 1780 RISVIKSVFS 1790 SRSLLYVGNR 1800 QASIIEYLPT 1810 LRNICKTEKL 1820 KEQGKSKRRF 1830 LHYFEGIHLD 1840 IPKETVNTLA ADFP

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0031391	Elg1 RFC-like complex
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0003677	DNA binding
Molecular Function	GO:0061860	DNA clamp unloader activity
Biological Process	GO:0008283	cell population proliferation
Biological Process	GO:0006974	DNA damage response
Biological Process	GO:1902166	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
Biological Process	GO:0033260	nuclear DNA replication
Biological Process	GO:1902751	positive regulation of cell cycle G2/M phase transition
Biological Process	GO:0045740	positive regulation of DNA replication

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.