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Overview

Uniprot IDQ96T21
Protein NameSelenocysteine insertion sequence-binding protein 2
Gene NameSECISBP2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
700 IISPNCEKIQSKGGL
704 NCEKIQSKGGLDDTL

Function

mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine (PubMed:35709277). Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling (PubMed:35709277). (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation (PubMed:35709277). (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur (PubMed:35709277)

Protein Sequence

10 MASEGPREPE 20 SEGIKLSADV 30 KPFVPRFAGL 40 NVAWLESSEA 50 CVFPSSAATY 60 YPFVQEPPVT 70 EQKIYTEDMA 80 FGASTFPPQY 90 LSSEITLHPY 100 AYSPYTLDST 110 QNVYSVPGSQ 120 YLYNQPSCYR 130 GFQTVKHRNE 140 NTCPLPQEMK 150 ALFKKKTYDE 160 KKTYDQQKFD 170 SERADGTISS 180 EIKSARGSHH 190 LSIYAENSLK 200 SDGYHKRTDR 210 KSRIIAKNVS 220 TSKPEFEFTT 230 LDFPELQGAE 240 NNMSEIQKQP 250 KWGPVHSVST 260 DISLLREVVK 270 PAAVLSKGEI 280 VVKNNPNESV 290 TANAATNSPS 300 CTRELSWTPM 310 GYVVRQTLST 320 ELSAAPKNVT 330 SMINLKTIAS 340 SADPKNVSIP 350 SSEALSSDPS 360 YNKEKHIIHP 370 TQKSKASQGS 380 DLEQNEASRK 390 NKKKKEKSTS 400 KYEVLTVQEP 410 PRIEDAEEFP 420 NLAVASERRD 430 RIETPKFQSK 440 QQPQDNFKNN 450 VKKSQLPVQL 460 DLGGMLTALE 470 KKQHSQHAKQ 480 SSKPVVVSVG 490 AVPVLSKECA 500 SGERGRRMSQ 510 MKTPHNPLDS 520 SAPLMKKGKQ 530 REIPKAKKPT 540 SLKKIILKER 550 QERKQRLQEN 560 AVSPAFTSDD 570 TQDGESGGDD 580 QFPEQAELSG 590 PEGMDELIST 600 PSVEDKSEEP 610 PGTELQRDTE 620 ASHLAPNHTT 630 FPKIHSRRFR 640 DYCSQMLSKE 650 VDACVTDLLK 660 ELVRFQDRMY 670 QKDPVKAKTK 680 RRLVLGLREV 690 LKHLKLKKLK 700 CVIISPNCEK 710 IQSKGGLDDT 720 LHTIIDYACE 730 QNIPFVFALN 740 RKALGRSLNK 750 AVPVSVVGIF 760 SYDGAQDQFH 770 KMVELTVAAR 780 QAYKTMLENV 790 QQELVGEPRP 800 QAPPSLPTQG 810 PSCPAEDGPP 820 ALKEKEEPHY 830 IEIWKKHLEA 840 YSGCTLELEE 850 SLEASTSQMM NLNL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:1990904 ribonucleoprotein complex
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003730 mRNA 3'-UTR binding
Molecular Function GO:0043021 ribonucleoprotein complex binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0035368 selenocysteine insertion sequence binding
Biological Process GO:0001514 selenocysteine incorporation

Reference

PMID: N/A