Search Results

Overview

Uniprot IDQ96T23
Protein NameRemodeling and spacing factor 1
Gene NameRSF1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1004 KKDSKKSKANLLERR
1050 GGGVGRGKDISTITG
1061 TITGHRGKDISTILD
1338 PSEQESTKKPYRIES
1339 SEQESTKKPYRIESD
1390 LIGKPTEKSQTPKDN
288 EDEKELVKLPVIVKL
294 VKLPVIVKLEKPLPE
323 KENVKPIKVEVKECR
358 IEFGGNIKSSHEITE
381 LKNDQQAKIPLKKRE
390 PLKKREIKLSDDFDS
400 DDFDSPVKGPLCKSV
405 PVKGPLCKSVTPTKE
439 TALGHEGKQLVNGEV
456 ERVAPNFKTEPIETK
511 KETAPLRKDADSSIS
577 SPKTKKDKRPPILEC
662 TVGGQSVKKVDLETL
663 VGGQSVKKVDLETLK
709 QKSKFKYKLVPEEET
787 RISRPTAKVAEIRDQ
959 QDLDVALKKKERAER

Function

Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:12972596, PubMed:28801535). Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed:12972596). Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively (PubMed:28801535). The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex (PubMed:28801535). The complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535). Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed:11788598, PubMed:11944984)

Protein Sequence

10 MATAAAAAAV 20 MAPPGCPGSC 30 PNFAVVCSFL 40 ERYGPLLDLP 50 ELPFPELERV 60 LQAPPPDVGN 70 GEVPKELVEL 80 HLKLMRKIGK 90 SVTADRWEKY 100 LIKICQEFNS 110 TWAWEMEKKG 120 YLEMSVECKL 130 ALLKYLCECQ 140 FDDNLKFKNI 150 INEEDADTMR 160 LQPIGRDKDG 170 LMYWYQLDQD 180 HNVRMYIEEQ 190 DDQDGSSWKC 200 IVRNRNELAE 210 TLALLKAQID 220 PVLLKNSSQQ 230 DNSSRESPSL 240 EDEETKKEEE 250 TPKQEEQKES 260 EKMKSEEQPM 270 DLENRSTANV 280 LEETTVKKEK 290 EDEKELVKLP 300 VIVKLEKPLP 310 ENEEKKIIKE 320 ESDSFKENVK 330 PIKVEVKECR 340 ADPKDTKSSM 350 EKPVAQEPER 360 IEFGGNIKSS 370 HEITEKSTEE 380 TEKLKNDQQA 390 KIPLKKREIK 400 LSDDFDSPVK 410 GPLCKSVTPT 420 KEFLKDEIKQ 430 EEETCKRIST 440 ITALGHEGKQ 450 LVNGEVSDER 460 VAPNFKTEPI 470 ETKFYETKEE 480 SYSPSKDRNI 490 ITEGNGTESL 500 NSVITSMKTG 510 ELEKETAPLR 520 KDADSSISVL 530 EIHSQKAQIE 540 EPDPPEMETS 550 LDSSEMAKDL 560 SSKTALSSTE 570 SCTMKGEEKS 580 PKTKKDKRPP 590 ILECLEKLEK 600 SKKTFLDKDA 610 QRLSPIPEEV 620 PKSTLESEKP 630 GSPEAAETSP 640 PSNIIDHCEK 650 LASEKEVVEC 660 QSTSTVGGQS 670 VKKVDLETLK 680 EDSEFTKVEM 690 DNLDNAQTSG 700 IEEPSETKGS 710 MQKSKFKYKL 720 VPEEETTASE 730 NTEITSERQK 740 EGIKLTIRIS 750 SRKKKPDSPP 760 KVLEPENKQE 770 KTEKEEEKTN 780 VGRTLRRSPR 790 ISRPTAKVAE 800 IRDQKADKKR 810 GEGEDEVEEE 820 STALQKTDKK 830 EILKKSEKDT 840 NSKVSKVKPK 850 GKVRWTGSRT 860 RGRWKYSSND 870 ESEGSGSEKS 880 SAASEEEEEK 890 ESEEAILADD 900 DEPCKKCGLP 910 NHPELILLCD 920 SCDSGYHTAC 930 LRPPLMIIPD 940 GEWFCPPCQH 950 KLLCEKLEEQ 960 LQDLDVALKK 970 KERAERRKER 980 LVYVGISIEN 990 IIPPQEPDFS 1000 EDQEEKKKDS 1010 KKSKANLLER 1020 RSTRTRKCIS 1030 YRFDEFDEAI 1040 DEAIEDDIKE 1050 ADGGGVGRGK 1060 DISTITGHRG 1070 KDISTILDEE 1080 RKENKRPQRA 1090 AAARRKKRRR 1100 LNDLDSDSNL 1110 DEEESEDEFK 1120 ISDGSQDEFV 1130 VSDENPDESE 1140 EDPPSNDDSD 1150 TDFCSRRLRR 1160 HPSRPMRQSR 1170 RLRRKTPKKK 1180 YSDDDEEEES 1190 EENSRDSESD 1200 FSDDFSDDFV 1210 ETRRRRSRRN 1220 QKRQINYKED 1230 SESDGSQKSL 1240 RRGKEIRRVH 1250 KRRLSSSESE 1260 ESYLSKNSED 1270 DELAKESKRS 1280 VRKRGRSTDE 1290 YSEADEEEEE 1300 EEGKPSRKRL 1310 HRIETDEEES 1320 CDNAHGDANQ 1330 PARDSQPRVL 1340 PSEQESTKKP 1350 YRIESDEEED 1360 FENVGKVGSP 1370 LDYSLVDLPS 1380 TNGQSPGKAI 1390 ENLIGKPTEK 1400 SQTPKDNSTA 1410 SASLASNGTS 1420 GGQEAGAPEE 1430 EEDELLRVTD 1440 LVDYVCNSEQ L

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0031213 RSF complex
Molecular Function GO:0042393 histone binding
Molecular Function GO:0140751 histone octamer slider activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006325 chromatin organization
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0006352 DNA-templated transcription initiation
Biological Process GO:0031507 heterochromatin formation
Biological Process GO:0043392 negative regulation of DNA binding
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0006334 nucleosome assembly
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0050434 positive regulation of viral transcription
Biological Process GO:0006355 regulation of DNA-templated transcription

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.