Search Results

Overview

Uniprot IDQ96T37
Protein NameRNA-binding protein 15
Gene NameRBM15
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
102 RRSLHLDKSSSRGGS
132 SYSSPSTKNSSGGGE
73 SRGERSKKLGGSGGS
762 STSTASSKLKSPSQK
764 STASSKLKSPSQKQD
769 KLKSPSQKQDGGTAP
87 SNGSSSGKTDSGGGS

Function

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed:27602518). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (By similarity). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (PubMed:27602518). Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation (By similarity). Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation; probably regulates alternative splicing via m6A regulation (PubMed:26575292). Required for placental vascular branching morphogenesis and embryonic development of the heart and spleen (By similarity). Acts as a regulator of thrombopoietin response in hematopoietic stem cells by regulating alternative splicing of MPL (By similarity). May also function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing (PubMed:17001072, PubMed:19786495). High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing (PubMed:17001072, PubMed:19786495). May be implicated in HOX gene regulation (PubMed:11344311)

Protein Sequence

10 MRTAGRDPVP 20 RRSPRWRRAV 30 PLCETSAGRR 40 VTQLRGDDLR 50 RPATMKGKER 60 SPVKAKRSRG 70 GEDSTSRGER 80 SKKLGGSGGS 90 NGSSSGKTDS 100 GGGSRRSLHL 110 DKSSSRGGSR 120 EYDTGGGSSS 130 SRLHSYSSPS 140 TKNSSGGGES 150 RSSSRGGGGE 160 SRSSGAASSA 170 PGGGDGAEYK 180 TLKISELGSQ 190 LSDEAVEDGL 200 FHEFKRFGDV 210 SVKISHLSGS 220 GSGDERVAFV 230 NFRRPEDARA 240 AKHARGRLVL 250 YDRPLKIEAV 260 YVSRRRSRSP 270 LDKDTYPPSA 280 SVVGASVGGH 290 RHPPGGGGGQ 300 RSLSPGGAAL 310 GYRDYRLQQL 320 ALGRLPPPPP 330 PPLPRDLERE 340 RDYPFYERVR 350 PAYSLEPRVG 360 AGAGAAPFRE 370 VDEISPEDDQ 380 RANRTLFLGN 390 LDITVTESDL 400 RRAFDRFGVI 410 TEVDIKRPSR 420 GQTSTYGFLK 430 FENLDMSHRA 440 KLAMSGKIII 450 RNPIKIGYGK 460 ATPTTRLWVG 470 GLGPWVPLAA 480 LAREFDRFGT 490 IRTIDYRKGD 500 SWAYIQYESL 510 DAAHAAWTHM 520 RGFPLGGPDR 530 RLRVDFADTE 540 HRYQQQYLQP 550 LPLTHYELVT 560 DAFGHRAPDP 570 LRGARDRTPP 580 LLYRDRDRDL 590 YPDSDWVPPP 600 PPVRERSTRT 610 AATSVPAYEP 620 LDSLDRRRDG 630 WSLDRDRGDR 640 DLPSSRDQPR 650 KRRLPEESGG 660 RHLDRSPESD 670 RPRKRHCAPS 680 PDRSPELSSS 690 RDRYNSDNDR 700 SSRLLLERPS 710 PIRDRRGSLE 720 KSQGDKRDRK 730 NSASAERDRK 740 HRTTAPTEGK 750 SPLKKEDRSD 760 GSAPSTSTAS 770 SKLKSPSQKQ 780 DGGTAPVASA 790 SPKLCLAWQG 800 MLLLKNSNFP 810 SNMHLLQGDL 820 QVASSLLVEG 830 STGGKVAQLK 840 ITQRLRLDQP 850 KLDEVTRRIK 860 VAGPNGYAIL 870 LAVPGSSDSR 880 SSSSSAASDT 890 ATSTQRPLRN 900 LVSYLKQKQA 910 AGVISLPVGG 920 NKDKENTGVL 930 HAFPPCEFSQ 940 QFLDSPAKAL 950 AKSEEDYLVM 960 IIVRGFGFQI 970 GVRYENKKRE NLALTLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0036396 RNA N6-methyladenosine methyltransferase complex
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0009048 dosage compensation by inactivation of X chromosome
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:0045652 regulation of megakaryocyte differentiation
Biological Process GO:0001510 RNA methylation
Biological Process GO:0038163 thrombopoietin-mediated signaling pathway

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.