Search Results

Overview

Uniprot ID	Q99490
Protein Name	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Gene Name	AGAP2
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
571	QKVVTLRKQQQLLAA

Function

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion

Protein Sequence

10 MSRGAGALQR 20 RTTTYLISLT 30 LVKLESVPPP 40 PPSPSAAAAG 50 AAGARGSETG 60 DPGSPRGAEE 70 PGKKRHERLF 80 HRQDALWIST 90 SSAGTGGAEP 100 PALSPAPASP 110 ARPVSPAPGR 120 RLSLWAVPPG 130 PPLSGGLSPD 140 PKPGGAPTSS 150 RRPLLSSPSW 160 GGPEPEGRAG 170 GGIPGSSSPH 180 PGTGSRRLKV 190 APPPPAPKPC 200 KTVTTSGAKA 210 GGGKGAGSRL 220 SWPESEGKPR 230 VKGSKSSAGT 240 GASVSAAATA 250 AAAGGGGSTA 260 STSGGVGAGA 270 GARGKLSPRK 280 GKSKTLDNSD 290 LHPGPPAGSP 300 PPLTLPPTPS 310 PATAVTAASA 320 QPPGPAPPIT 330 LEPPAPGLKR 340 GREGGRASTR 350 DRKMLKFISG 360 IFTKSTGGPP 370 GSGPLPGPPS 380 LSSGSGSREL 390 LGAELRASPK 400 AVINSQEWTL 410 SRSIPELRLG 420 VLGDARSGKS 430 SLIHRFLTGS 440 YQVLEKTESE 450 QYKKEMLVDG 460 QTHLVLIREE 470 AGAPDAKFSG 480 WADAVIFVFS 490 LEDENSFQAV 500 SRLHGQLSSL 510 RGEGRGGLAL 520 ALVGTQDRIS 530 ASSPRVVGDA 540 RARALCADMK 550 RCSYYETCAT 560 YGLNVDRVFQ 570 EVAQKVVTLR 580 KQQQLLAACK 590 SLPSSPSHSA 600 ASTPVAGQAS 610 NGGHTSDYSS 620 SLPSSPNVGH 630 RELRAEAAAV 640 AGLSTPGSLH 650 RAAKRRTSLF 660 ANRRGSDSEK 670 RSLDSRGETT 680 GSGRAIPIKQ 690 SFLLKRSGNS 700 LNKEWKKKYV 710 TLSSNGFLLY 720 HPSINDYIHS 730 THGKEMDLLR 740 TTVKVPGKRP 750 PRAISAFGPS 760 ASINGLVKDM 770 STVQMGEGLE 780 ATTPMPSPSP 790 SPSSLQPPPD 800 QTSKHLLKPD 810 RNLARALSTD 820 CTPSGDLSPL 830 SREPPPSPMV 840 KKQRRKKLTT 850 PSKTEGSAGQ 860 AEAKRKMWKL 870 KSFGSLRNIY 880 KAEENFEFLI 890 VSSTGQTWHF 900 EAASFEERDA 910 WVQAIESQIL 920 ASLQCCESSK 930 VKLRTDSQSE 940 AVAIQAIRNA 950 KGNSICVDCG 960 APNPTWASLN 970 LGALICIECS 980 GIHRNLGTHL 990 SRVRSLDLDD 1000 WPRELTLVLT 1010 AIGNDTANRV 1020 WESDTRGRAK 1030 PSRDSSREER 1040 ESWIRAKYEQ 1050 LLFLAPLSTS 1060 EEPLGRQLWA 1070 AVQAQDVATV 1080 LLLLAHARHG 1090 PLDTSVEDPQ 1100 LRSPLHLAAE 1110 LAHVVITQLL 1120 LWYGADVAAR 1130 DAQGRTALFY 1140 ARQAGSQLCA 1150 DILLQHGCPG 1160 EGGSAATTPS 1170 AATTPSITAT 1180 PSPRRRSSAA 1190 SVGRADAPVA LV

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005768	endosome
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0005096	GTPase activator activity
Molecular Function	GO:0003924	GTPase activity
Molecular Function	GO:0141038	phosphatidylinositol 3-kinase activator activity
Molecular Function	GO:0035014	phosphatidylinositol 3-kinase regulator activity
Molecular Function	GO:0030295	protein kinase activator activity
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0030036	actin cytoskeleton organization
Biological Process	GO:0016197	endosomal transport
Biological Process	GO:0043524	negative regulation of neuron apoptotic process
Biological Process	GO:0042177	negative regulation of protein catabolic process
Biological Process	GO:0045860	positive regulation of protein kinase activity
Biological Process	GO:0015031	protein transport

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.