Overview
| Uniprot ID | Q99496 |
|---|
| Protein Name | E3 ubiquitin-protein ligase RING2 |
|---|
| Gene Name | RNF2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 133 |
RVLARINKHNNQQAL |
Function
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15386022, PubMed:16359901, PubMed:21772249, PubMed:25355358, PubMed:25519132, PubMed:26151332, PubMed:33864376). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:16359901, PubMed:26151332). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:26151332). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:21772249). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (By similarity). Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation (By similarity)
Protein Sequence
10
MSQAVQTNGT
20
QPLSKTWELS
30
LYELQRTPQE
40
AITDGLEIVV
50
SPRSLHSELM
60
CPICLDMLKN
70
TMTTKECLHR
80
FCADCIITAL
90
RSGNKECPTC
100
RKKLVSKRSL
110
RPDPNFDALI
120
SKIYPSRDEY
130
EAHQERVLAR
140
INKHNNQQAL
150
SHSIEEGLKI
160
QAMNRLQRGK
170
KQQIENGSGA
180
EDNGDSSHCS
190
NASTHSNQEA
200
GPSNKRTKTS
210
DDSGLELDNN
220
NAAMAIDPVM
230
DGASEIELVF
240
RPHPTLMEKD
250
DSAQTRYIKT
260
SGNATVDHLS
270
KYLAVRLALE
280
ELRSKGESNQ
290
MNLDTASEKQ
300
YTIYIATASG
310
QFTVLNGSFS
320
LELVSEKYWK
330
VNKPMELYYA
PTKEHK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0000791 |
euchromatin |
| Cellular Component |
GO:0071339 |
MLL1 complex |
| Cellular Component |
GO:0016604 |
nuclear body |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0031519 |
PcG protein complex |
| Cellular Component |
GO:0035102 |
PRC1 complex |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Cellular Component |
GO:0001739 |
sex chromatin |
| Cellular Component |
GO:0000151 |
ubiquitin ligase complex |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0140862 |
histone H2AK119 ubiquitin ligase activity |
| Molecular Function |
GO:0071535 |
RING-like zinc finger domain binding |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006338 |
chromatin remodeling |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0040029 |
epigenetic regulation of gene expression |
| Biological Process |
GO:0007281 |
germ cell development |
| Biological Process |
GO:0031507 |
heterochromatin formation |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0045893 |
positive regulation of DNA-templated transcription |
| Biological Process |
GO:0016567 |
protein ubiquitination |
Reference
PMID: N/A