Search Results
Overview
| Uniprot ID | Q99714 |
|---|---|
| Protein Name | 3-hydroxyacyl-CoA dehydrogenase type-2 |
| Gene Name | HSD17B10 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 105 | SKTYNLKKGQTHTLE |
| 52 | SGGEAQAKKLGNNCV |
| 53 | GGEAQAKKLGNNCVF |
| 79 | QTALALAKGKFGRVD |
| 9 | AAACRSVKGLVAVIT |
| 99 | AGIAVASKTYNLKKG |
Function
Mitochondrial dehydrogenase involved in pathways of fatty acid, branched-chain amino acid and steroid metabolism (PubMed:10600649, PubMed:12917011, PubMed:18996107, PubMed:19706438, PubMed:20077426, PubMed:25925575, PubMed:26950678, PubMed:28888424, PubMed:9553139). Acts as (S)-3-hydroxyacyl-CoA dehydrogenase in mitochondrial fatty acid beta-oxidation, a major degradation pathway of fatty acids. Catalyzes the third step in the beta-oxidation cycle, namely the reversible conversion of (S)-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. Preferentially accepts straight medium- and short-chain acyl-CoA substrates with highest efficiency for (3S)-hydroxybutanoyl-CoA (PubMed:10600649, PubMed:12917011, PubMed:25925575, PubMed:26950678, PubMed:9553139). Acts as 3-hydroxy-2-methylbutyryl-CoA dehydrogenase in branched-chain amino acid catabolic pathway. Catalyzes the oxidation of 3-hydroxy-2-methylbutanoyl-CoA into 2-methyl-3-oxobutanoyl-CoA, a step in isoleucine degradation pathway (PubMed:18996107, PubMed:19706438, PubMed:20077426). Has hydroxysteroid dehydrogenase activity toward steroid hormones and bile acids. Catalyzes the oxidation of 3alpha-, 17beta-, 20beta- and 21-hydroxysteroids and 7alpha- and 7beta-hydroxy bile acids (PubMed:10600649, PubMed:12917011). Oxidizes allopregnanolone/brexanolone at the 3alpha-hydroxyl group, which is known to be critical for the activation of gamma-aminobutyric acid receptors (GABAARs) chloride channel (PubMed:19706438, PubMed:28888424). Has phospholipase C-like activity toward cardiolipin and its oxidized species. Likely oxidizes the 2'-hydroxyl in the head group of cardiolipin to form a ketone intermediate that undergoes nucleophilic attack by water and fragments into diacylglycerol, dihydroxyacetone and orthophosphate. Has higher affinity for cardiolipin with oxidized fatty acids and may degrade these species during the oxidative stress response to protect cells from apoptosis (PubMed:26338420). By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD) (PubMed:9338779). Essential for structural and functional integrity of mitochondria (PubMed:20077426)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0042645 | mitochondrial nucleoid |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005759 | mitochondrial matrix |
| Cellular Component | GO:0030678 | mitochondrial ribonuclease P complex |
| Cellular Component | GO:0005739 | mitochondrion |
| Cellular Component | GO:0005886 | plasma membrane |
| Molecular Function | GO:0003857 | (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| Molecular Function | GO:0044594 | 17-beta-hydroxysteroid dehydrogenase (NAD+) activity |
| Molecular Function | GO:0047015 | 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity |
| Molecular Function | GO:0047044 | androstan-3-alpha,17-beta-diol dehydrogenase (NAD+) activity |
| Molecular Function | GO:0160241 | cardiolipin dehydrogenase (NAD+) activity |
| Molecular Function | GO:0106281 | chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity |
| Molecular Function | GO:0008709 | cholate 7-alpha-dehydrogenase (NAD+) activity |
| Molecular Function | GO:0004303 | estradiol 17-beta-dehydrogenase [NAD(P)+] activity |
| Molecular Function | GO:0106282 | isoursodeoxycholate 7-beta-dehydrogenase (NAD+) activity |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0047035 | testosterone dehydrogenase (NAD+) activity |
| Molecular Function | GO:0000049 | tRNA binding |
| Molecular Function | GO:0106283 | ursodeoxycholate 7-beta-dehydrogenase (NAD+) activity |
| Biological Process | GO:0008209 | androgen metabolic process |
| Biological Process | GO:0006699 | bile acid biosynthetic process |
| Biological Process | GO:0062173 | brexanolone metabolic process |
| Biological Process | GO:0008207 | C21-steroid hormone metabolic process |
| Biological Process | GO:0008210 | estrogen metabolic process |
| Biological Process | GO:0006635 | fatty acid beta-oxidation |
| Biological Process | GO:0006631 | fatty acid metabolic process |
| Biological Process | GO:0006550 | L-isoleucine catabolic process |
| Biological Process | GO:0006629 | lipid metabolic process |
| Biological Process | GO:1990180 | mitochondrial tRNA 3'-end processing |
| Biological Process | GO:0097745 | mitochondrial tRNA 5'-end processing |
| Biological Process | GO:0070901 | mitochondrial tRNA methylation |
| Biological Process | GO:0007005 | mitochondrion organization |
| Biological Process | GO:0051289 | protein homotetramerization |
| Biological Process | GO:0042780 | tRNA 3'-end processing |
| Biological Process | GO:0001680 | tRNA 3'-terminal CCA addition |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.
[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.
[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.
[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.