Overview
| Uniprot ID | Q99K48 |
|---|
| Protein Name | Non-POU domain-containing octamer-binding protein |
|---|
| Gene Name | Nono |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 338 |
LHNQEVQKRKQLELR |
Function
DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA (PubMed:8355702). Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ (By similarity). Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b (By similarity). Together with PSPC1, required for the formation of nuclear paraspeckles (By similarity). The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs (By similarity). The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 (By similarity). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends (By similarity). In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex (By similarity). NONO is involved in transcriptional regulation (By similarity). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity (By similarity). NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (PubMed:9001221). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (PubMed:22966205). Important for the functional organization of GABAergic synapses (PubMed:26571461). Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript (PubMed:26571461). Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels (PubMed:32286661). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity)
Protein Sequence
10
MQSNKAFNLE
20
KQNHTPRKHH
30
QHHHQQHHQQ
40
QQQQQQQQPP
50
PPIPANGQQA
60
SSQNEGLTID
70
LKNFRKPGEK
80
TFTQRSRLFV
90
GNLPPDITEE
100
EMRKLFEKYG
110
KAGEVFIHKD
120
KGFGFIRLET
130
RTLAEIAKVE
140
LDNMPLRGKQ
150
LRVRFACHSA
160
SLTVRNLPQY
170
VSNELLEEAF
180
SVFGQVERAV
190
VIVDDRGRPS
200
GKGIVEFSGK
210
PAARKALDRC
220
SEGSFLLTTF
230
PRPVTVEPMD
240
QLDDEEGLPE
250
KLVIKNQQFH
260
KEREQPPRFA
270
QPGSFEYEYA
280
MRWKALIEME
290
KQQQDQVDRN
300
IKEAREKLEM
310
EMEAARHEHQ
320
VMLMRQDLMR
330
RQEELRRMEE
340
LHNQEVQKRK
350
QLELRQEEER
360
RRREEEMRRQ
370
QEEMMRRQQE
380
GFKGTFPDAR
390
EQEIRMGQMA
400
MGGAMGINNR
410
GAMPPAPVPP
420
GTPAPPGPAT
430
MMPDGTLGLT
440
PPTTERFGQA
450
ATMEGIGAIG
460
GTPPAFNRPA
470
PGAEFAPNKR
RRY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0001650 |
fibrillar center |
| Cellular Component |
GO:0016363 |
nuclear matrix |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0042382 |
paraspeckles |
| Cellular Component |
GO:0090575 |
RNA polymerase II transcription regulator complex |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0106222 |
lncRNA binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0002218 |
activation of innate immune response |
| Biological Process |
GO:1904385 |
cellular response to angiotensin |
| Biological Process |
GO:0071456 |
cellular response to hypoxia |
| Biological Process |
GO:0007623 |
circadian rhythm |
| Biological Process |
GO:0006310 |
DNA recombination |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0043066 |
negative regulation of apoptotic process |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:1903377 |
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| Biological Process |
GO:0042752 |
regulation of circadian rhythm |
| Biological Process |
GO:0006355 |
regulation of DNA-templated transcription |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.