Search Results

Overview

Uniprot IDQ99KR7
Protein NamePeptidyl-prolyl cis-trans isomerase F, mitochondrial
Gene NamePpif
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
166 KTDWLDGKHVVFGHV
174 HVVFGHVKEGMDVVK
189 KIESFGSKSGKTSKK
66 GRVVLELKADVVPKT
72 LKADVVPKTAENFRA
85 RALCTGEKGFGYKGS
90 GEKGFGYKGSTFHRV

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (By similarity). Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed:15800626, PubMed:15800627, PubMed:16103352, PubMed:18684715, PubMed:31489369). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed:15800626, PubMed:15800627, PubMed:16103352, PubMed:18684715, PubMed:31489369). In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed:22726440). Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (PubMed:19801635, PubMed:21281446). Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (By similarity)

Protein Sequence

10 MLALRCGPRL 20 LGLLSGPRSA 30 PLLLSATRTC 40 SDGGARGANS 50 SSGNPLVYLD 60 VGADGQPLGR 70 VVLELKADVV 80 PKTAENFRAL 90 CTGEKGFGYK 100 GSTFHRVIPA 110 FMCQAGDFTN 120 HNGTGGRSIY 130 GSRFPDENFT 140 LKHVGPGVLS 150 MANAGPNTNG 160 SQFFICTIKT 170 DWLDGKHVVF 180 GHVKEGMDVV 190 KKIESFGSKS 200 GKTSKKIVIT DCGQLS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005757 mitochondrial permeability transition pore complex
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0016018 cyclosporin A binding
Molecular Function GO:0004857 enzyme inhibitor activity
Molecular Function GO:0042277 peptide binding
Molecular Function GO:0003755 peptidyl-prolyl cis-trans isomerase activity
Biological Process GO:0008637 apoptotic mitochondrial changes
Biological Process GO:0006915 apoptotic process
Biological Process GO:0071243 cellular response to arsenic-containing substance
Biological Process GO:0071277 cellular response to calcium ion
Biological Process GO:0070301 cellular response to hydrogen peroxide
Biological Process GO:0051882 mitochondrial depolarization
Biological Process GO:1902686 mitochondrial outer membrane permeabilization involved in programmed cell death
Biological Process GO:0007005 mitochondrion organization
Biological Process GO:0061061 muscle structure development
Biological Process GO:0070266 necroptotic process
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:2001243 negative regulation of intrinsic apoptotic signaling pathway
Biological Process GO:0090324 negative regulation of oxidative phosphorylation
Biological Process GO:0090201 negative regulation of release of cytochrome c from mitochondria
Biological Process GO:0012501 programmed cell death
Biological Process GO:0006457 protein folding
Biological Process GO:0042981 regulation of apoptotic process
Biological Process GO:0046902 regulation of mitochondrial membrane permeability
Biological Process GO:1902445 regulation of mitochondrial membrane permeability involved in programmed necrotic cell death
Biological Process GO:0002931 response to ischemia
Biological Process GO:0006979 response to oxidative stress
Biological Process GO:0098528 skeletal muscle fiber differentiation

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.