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Overview

Uniprot IDQ99M87
Protein NameDnaJ homolog subfamily A member 3, mitochondrial
Gene NameDnaja3
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
130 DTNKDDPKAKEKFSQ
335 FVTFRVQKSPVFRRD

Function

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway

Protein Sequence

10 MAAWCSPRWL 20 RVAVGTPRLP 30 AAAGRGVQQP 40 QGGVVATSLC 50 RKLCVSAFGL 60 SMGAHGPRAL 70 LTLRPGVRLT 80 GTKSFPFVCT 90 TSFHTSASLA 100 KDDYYQILGV 110 PRNASQKDIK 120 KAYYQLAKKY 130 HPDTNKDDPK 140 AKEKFSQLAE 150 AYEVLSDEVK 160 RKQYDAYGSA 170 GFDPGTSSSG 180 QGYWRGGPSV 190 DPEELFRKIF 200 GEFSSSPFGD 210 FQNVFDQPQE 220 YIMELTFNQA 230 AKGVNKEFTV 240 NIMDTCERCD 250 GKGNEPGTKV 260 QHCHYCGGSG 270 METINTGPFV 280 MRSTCRRCGG 290 RGSIITNPCV 300 VCRGAGQAKQ 310 KKRVTIPVPA 320 GVEDGQTVRM 330 PVGKREIFVT 340 FRVQKSPVFR 350 RDGADIHSDL 360 FISIAQAILG 370 GTAKAQGLYE 380 TINVTIPAGI 390 QTDQKIRLTG 400 KGIPRINSYG 410 YGDHYIHIKI 420 RVPKRLSSRQ 430 QNLILSYAED 440 ETDVEGTVNG 450 VTHTSTGGRT 460 MDSSAGSKDR 470 REAGEDNEGF 480 LSKLKKIFTS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005884 actin filament
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0009898 cytoplasmic side of plasma membrane
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0042645 mitochondrial nucleoid
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0031594 neuromuscular junction
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0045211 postsynaptic membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0140297 DNA-binding transcription factor binding
Molecular Function GO:0030695 GTPase regulator activity
Molecular Function GO:0030544 Hsp70 protein binding
Molecular Function GO:0106137 IkappaB kinase complex binding
Molecular Function GO:0051059 NF-kappaB binding
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0030971 receptor tyrosine kinase binding
Molecular Function GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
Molecular Function GO:0005133 type II interferon receptor binding
Molecular Function GO:0051082 unfolded protein binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006924 activation-induced cell death of T cells
Biological Process GO:0006915 apoptotic process
Biological Process GO:0090398 cellular senescence
Biological Process GO:0006264 mitochondrial DNA replication
Biological Process GO:0007005 mitochondrion organization
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0043124 negative regulation of canonical NF-kappaB signal transduction
Biological Process GO:0008285 negative regulation of cell population proliferation
Biological Process GO:0043069 negative regulation of programmed cell death
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0060336 negative regulation of type II interferon-mediated signaling pathway
Biological Process GO:0007528 neuromuscular junction development
Biological Process GO:0043065 positive regulation of apoptotic process
Biological Process GO:0031398 positive regulation of protein ubiquitination
Biological Process GO:0042102 positive regulation of T cell proliferation
Biological Process GO:0006457 protein folding
Biological Process GO:0050821 protein stabilization
Biological Process GO:0009408 response to heat
Biological Process GO:0034341 response to type II interferon
Biological Process GO:0071340 skeletal muscle acetylcholine-gated channel clustering
Biological Process GO:0007264 small GTPase-mediated signal transduction
Biological Process GO:0033077 T cell differentiation in thymus

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.