Search Results

Overview

Uniprot ID	Q99NB1
Protein Name	Acetyl-coenzyme A synthetase 2-like, mitochondrial
Gene Name	Acss1
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
151	CRLANTLKRHGVHRG
233	KIVDEAVKSCPTVQH
389	TAVRLLLKYGDAWVK
615	SVATKIAKYAVPDQI
626	PDQILVVKRLPKTRS

Function

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:11150295, PubMed:16790548). Acetate is the preferred substrate (PubMed:11150295, PubMed:16790548). Can also utilize propionate with a much lower affinity (PubMed:11150295). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (PubMed:11150295). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (PubMed:19187775)

Protein Sequence

10 MAARSLGSGV 20 GRLLRGLQGR 30 SGQSGWSLSV 40 SRSTATRLPG 50 CVPAAAQPGS 60 YPALSAQAAQ 70 EPAAFWGPLA 80 RDTLVWDTPY 90 HTVWDCDFRT 100 GKIGWFLGGQ 110 LNVSVNCLDQ 120 HVQKSPETIA 130 LIWERDEPGT 140 EVRITYRELL 150 ETTCRLANTL 160 KRHGVHRGDR 170 VAIYMPVSPL 180 AVAAMLACAR 190 IGAIHTVVFA 200 GFSAESLAGR 210 INDAKCKAVI 220 TFNQGLRGGR 230 VVELKKIVDE 240 AVKSCPTVQH 250 VLVAHRTDTK 260 VPMGSLDIPL 270 EQEMAKEAPV 280 CTPESMSSED 290 MLFMLYTSGS 300 TGTPKGLVHT 310 QAGYLLYAAM 320 THKLVFDYQP 330 GDVFGCVADI 340 GWITGHSYVV 350 YGPLCNGATT 360 VLFESTPVYP 370 DAGRYWETVQ 380 RLKINQFYGA 390 PTAVRLLLKY 400 GDAWVKKYDR 410 SSLRTLGSVG 420 EPINHEAWEW 430 LHKVVGDGRC 440 TLVDTWWQTE 450 TGGICIAPRP 460 SEDGAEILPG 470 MAMRPFFGIV 480 PVLMDEKGNV 490 LEGGDVSGAL 500 CISQAWPGMA 510 RTIYGDHQRF 520 VDAYFRAYPG 530 YYFTGDGAHR 540 TEGGYYQITG 550 RMDDVINISG 560 HRLGTAEIED 570 AMADHPAVPE 580 TAVIGYPHDI 590 KGEAAFAFIV 600 LKDNISDENM 610 VVNELKLSVA 620 TKIAKYAVPD 630 QILVVKRLPK 640 TRSGKVMRRL 650 LRKIITSRGQ 660 DLGDTTTLED 670 PSVITEILSA 680 FQKYEEQRAA TN

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0003987	acetate-CoA ligase activity
Molecular Function	GO:0016208	AMP binding
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0050218	propionate-CoA ligase activity
Biological Process	GO:0019413	acetate biosynthetic process
Biological Process	GO:0006085	acetyl-CoA biosynthetic process
Biological Process	GO:0019427	acetyl-CoA biosynthetic process from acetate
Biological Process	GO:0006068	ethanol catabolic process
Biological Process	GO:0019542	propionate biosynthetic process

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.