Search Results

Overview

Uniprot IDQ9BTA9
Protein NameWW domain-containing adapter protein with coiled-coil
Gene NameWAC
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
175 QRQKEANKMAVNSFP
183 MAVNSFPKDRDYRRE
29 SQPYQALKYSSKSHP
302 TSSVPAQKTERKEST
33 QALKYSSKSHPSSGD
486 KVSTPVVKQGPVSQS
57 GDPSPPNKMLRRSDS
77 SDSTGHSKAKNVHTH
79 STGHSKAKNVHTHRV

Function

Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1) (PubMed:21329877). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription (PubMed:21329877). Regulates the cell-cycle checkpoint activation in response to DNA damage (PubMed:21329877). Positive regulator of amino acid starvation-induced autophagy (PubMed:22354037). Also acts as a negative regulator of basal autophagy (PubMed:26812014). Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation (PubMed:26812014). May negatively regulate the ubiquitin proteasome pathway (PubMed:21329877)

Protein Sequence

10 MVMYARKQQR 20 LSDGCHDRRG 30 DSQPYQALKY 40 SSKSHPSSGD 50 HRHEKMRDAG 60 DPSPPNKMLR 70 RSDSPENKYS 80 DSTGHSKAKN 90 VHTHRVRERD 100 GGTSYSPQEN 110 SHNHSALHSS 120 NSHSSNPSNN 130 PSKTSDAPYD 140 SADDWSEHIS 150 SSGKKYYYNC 160 RTEVSQWEKP 170 KEWLEREQRQ 180 KEANKMAVNS 190 FPKDRDYRRE 200 VMQATATSGF 210 ASGMEDKHSS 220 DASSLLPQNI 230 LSQTSRHNDR 240 DYRLPRAETH 250 SSSTPVQHPI 260 KPVVHPTATP 270 STVPSSPFTL 280 QSDHQPKKSF 290 DANGASTLSK 300 LPTPTSSVPA 310 QKTERKESTS 320 GDKPVSHSCT 330 TPSTSSASGL 340 NPTSAPPTSA 350 SAVPVSPVPQ 360 SPIPPLLQDP 370 NLLRQLLPAL 380 QATLQLNNSN 390 VDISKINEVL 400 TAAVTQASLQ 410 SIIHKFLTAG 420 PSAFNITSLI 430 SQAAQLSTQA 440 QPSNQSPMSL 450 TSDASSPRSY 460 VSPRISTPQT 470 NTVPIKPLIS 480 TPPVSSQPKV 490 STPVVKQGPV 500 SQSATQQPVT 510 ADKQQGHEPV 520 SPRSLQRSSS 530 QRSPSPGPNH 540 TSNSSNASNA 550 TVVPQNSSAR 560 STCSLTPALA 570 AHFSENLIKH 580 VQGWPADHAE 590 KQASRLREEA 600 HNMGTIHMSE 610 ICTELKNLRS 620 LVRVCEIQAT 630 LREQRILFLR 640 QQIKELEKLK NQNSFMV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0000993 RNA polymerase II complex binding
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0006974 DNA damage response
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0031571 mitotic G1 DNA damage checkpoint signaling
Biological Process GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0016239 positive regulation of macroautophagy
Biological Process GO:1904263 positive regulation of TORC1 signaling
Biological Process GO:0010506 regulation of autophagy

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.