Search Results

Overview

Uniprot IDQ9BTZ2
Protein NameDehydrogenase/reductase SDR family member 4
Gene NameDHRS4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
158 AVVPEMEKRGGGSVV
227 SRMLWMDKEKEESMK
229 MLWMDKEKEESMKET
234 KEKEESMKETLRIRR
65 HVVVSSRKQQNVDQA
92 GTVCHVGKAEDRERL

Function

NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones (PubMed:18571493, PubMed:19056333). Reduces 3-ketosteroids and benzil into 3beta-hydroxysteroids and R-benzoin, respectively, in contrast to the stereoselectivity of non-primate DHRS4s which produce 3alpha-hydroxysteroids and S-benzoin (PubMed:19056333). Displays low activity toward all-trans-retinal and no activity toward 9-cis-retinal as compared to non-primate mammals (PubMed:18571493, PubMed:19056333). In the reverse reaction, catalyze the NAD-dependent oxidation of 3beta-hydroxysteroids and alcohol, but with much lower efficiency (PubMed:18571493, PubMed:19056333). Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds (PubMed:18571493, PubMed:19056333)

Protein Sequence

10 MHKAGLLGLC 20 ARAWNSVRMA 30 SSGMTRRDPL 40 ANKVALVTAS 50 TDGIGFAIAR 60 RLAQDGAHVV 70 VSSRKQQNVD 80 QAVATLQGEG 90 LSVTGTVCHV 100 GKAEDRERLV 110 ATAVKLHGGI 120 DILVSNAAVN 130 PFFGSIMDVT 140 EEVWDKTLDI 150 NVKAPALMTK 160 AVVPEMEKRG 170 GGSVVIVSSI 180 AAFSPSPGFS 190 PYNVSKTALL 200 GLTKTLAIEL 210 APRNIRVNCL 220 APGLIKTSFS 230 RMLWMDKEKE 240 ESMKETLRIR 250 RLGEPEDCAG 260 IVSFLCSEDA 270 SYITGETVVV GGGTPSRL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005782 peroxisomal matrix
Cellular Component GO:0005778 peroxisomal membrane
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0033703 3-beta-hydroxy-5-beta-steroid dehydrogenase (NADP+) activity
Molecular Function GO:0000253 3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity
Molecular Function GO:0018455 alcohol dehydrogenase [NAD(P)+] activity
Molecular Function GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity
Molecular Function GO:0004090 carbonyl reductase (NADPH) activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
Biological Process GO:0006066 alcohol metabolic process
Biological Process GO:0042180 ketone metabolic process
Biological Process GO:2000379 positive regulation of reactive oxygen species metabolic process
Biological Process GO:0042574 retinal metabolic process
Biological Process GO:0008202 steroid metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.