Overview
| Uniprot ID | Q9BUI4 |
|---|
| Protein Name | DNA-directed RNA polymerase III subunit RPC3 |
|---|
| Gene Name | POLR3C |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 198 |
PKLSLIGKGKRRRSS |
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:33558764, PubMed:33558766, PubMed:34675218, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:20413673, PubMed:33558764, PubMed:33558766, PubMed:35637192). Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer, coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state (PubMed:33558764, PubMed:33558766). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370). Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628)
Protein Sequence
10
MTQAEIKLCS
20
LLLQEHFGEI
30
VEKIGVHLIR
40
TGSQPLRVIA
50
HDTGTSLDQV
60
KKALCVLVQH
70
NLVSYQVHKR
80
GVVEYEAQCS
90
RVLRMLRYPR
100
YIYTTKTLYS
110
DTGELIVEEL
120
LLNGKLTMSA
130
VVKKVADRLT
140
ETMEDGKTMD
150
YAEVSNTFVR
160
LADTHFVQRC
170
PSVPTTENSD
180
PGPPPPAPTL
190
VINEKDMYLV
200
PKLSLIGKGK
210
RRRSSDEDAA
220
GEPKAKRPKY
230
TTDNKEPIPD
240
DGIYWQANLD
250
RFHQHFRDQA
260
IVSAVANRMD
270
QTSSEIVRTM
280
LRMSEITTSS
290
SAPFTQPLSS
300
NEIFRSLPVG
310
YNISKQVLDQ
320
YLTLLADDPL
330
EFVGKSGDSG
340
GGMYVINLHK
350
ALASLATATL
360
ESVVQERFGS
370
RCARIFRLVL
380
QKKHIEQKQV
390
EDFAMIPAKE
400
AKDMLYKMLS
410
ENFMSLQEIP
420
KTPDHAPSRT
430
FYLYTVNILS
440
AARMLLHRCY
450
KSIANLIERR
460
QFETKENKRL
470
LEKSQRVEAI
480
IASMQATGAE
490
EAQLQEIEEM
500
ITAPERQQLE
510
TLKRNVNKLD
520
ASEIQVDETI
530
FLLESYIECT
MKRQ
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005666 |
RNA polymerase III complex |
| Molecular Function |
GO:0003899 |
DNA-directed RNA polymerase activity |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Biological Process |
GO:0051607 |
defense response to virus |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0045089 |
positive regulation of innate immune response |
| Biological Process |
GO:0032728 |
positive regulation of interferon-beta production |
| Biological Process |
GO:0006359 |
regulation of transcription by RNA polymerase III |
| Biological Process |
GO:0006386 |
termination of RNA polymerase III transcription |
| Biological Process |
GO:0006383 |
transcription by RNA polymerase III |
| Biological Process |
GO:0006384 |
transcription initiation at RNA polymerase III promoter |
Reference
PMID: N/A