Search Results

Overview

Uniprot IDQ9BUQ8
Protein NameProbable ATP-dependent RNA helicase DDX23
Gene NameDDX23
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
196 EEERKKRKQFQDLGR
259 ERYLGGIKKRRRTRH
260 RYLGGIKKRRRTRHL
310 FIAGIDLKQQKREQS
698 ACTLHGGKGQEQREF
711 EFALSNLKAGAKDIL
811 KPGTILTKKRREETI
812 PGTILTKKRREETIF

Function

Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation (PubMed:18425142). Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:28076779)

Protein Sequence

10 MAGELADKKD 20 RDASPSKEER 30 KRSRTPDRER 40 DRDRDRKSSP 50 SKDRKRHRSR 60 DRRRGGSRSR 70 SRSRSKSAER 80 ERRHKERERD 90 KERDRNKKDR 100 DRDKDGHRRD 110 KDRKRSSLSP 120 GRGKDFKSRK 130 DRDSKKDEED 140 EHGDKKPKAQ 150 PLSLEELLAK 160 KKAEEEAEAK 170 PKFLSKAERE 180 AEALKRRQQE 190 VEERQRMLEE 200 ERKKRKQFQD 210 LGRKMLEDPQ 220 ERERRERRER 230 MERETNGNED 240 EEGRQKIREE 250 KDKSKELHAI 260 KERYLGGIKK 270 RRRTRHLNDR 280 KFVFEWDASE 290 DTSIDYNPLY 300 KERHQVQLLG 310 RGFIAGIDLK 320 QQKREQSRFY 330 GDLMEKRRTL 340 EEKEQEEARL 350 RKLRKKEAKQ 360 RWDDRHWSQK 370 KLDEMTDRDW 380 RIFREDYSIT 390 TKGGKIPNPI 400 RSWKDSSLPP 410 HILEVIDKCG 420 YKEPTPIQRQ 430 AIPIGLQNRD 440 IIGVAETGSG 450 KTAAFLIPLL 460 VWITTLPKID 470 RIEESDQGPY 480 AIILAPTREL 490 AQQIEEETIK 500 FGKPLGIRTV 510 AVIGGISRED 520 QGFRLRMGCE 530 IVIATPGRLI 540 DVLENRYLVL 550 SRCTYVVLDE 560 ADRMIDMGFE 570 PDVQKILEHM 580 PVSNQKPDTD 590 EAEDPEKMLA 600 NFESGKHKYR 610 QTVMFTATMP 620 PAVERLARSY 630 LRRPAVVYIG 640 SAGKPHERVE 650 QKVFLMSESE 660 KRKKLLAILE 670 QGFDPPIIIF 680 VNQKKGCDVL 690 AKSLEKMGYN 700 ACTLHGGKGQ 710 EQREFALSNL 720 KAGAKDILVA 730 TDVAGRGIDI 740 QDVSMVVNYD 750 MAKNIEDYIH 760 RIGRTGRAGK 770 SGVAITFLTK 780 EDSAVFYELK 790 QAILESPVSS 800 CPPELANHPD 810 AQHKPGTILT 820 KKRREETIFA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0000785 chromatin
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex
Cellular Component GO:0005682 U5 snRNP
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0000354 cis assembly of pre-catalytic spliceosome
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0062176 R-loop processing
Biological Process GO:0008380 RNA splicing
Biological Process GO:0000375 RNA splicing, via transesterification reactions
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.