Overview
| Uniprot ID | Q9BV57 |
|---|
| Protein Name | Acireductone dioxygenase |
|---|
| Gene Name | ADI1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 140 |
HRFTVDEKNYTKAMR |
| 73 |
IITICKDKLPNYEEK |
Function
Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site (By similarity). Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway (PubMed:15938715). Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway (By similarity). Also down-regulates cell migration mediated by MMP14 (PubMed:14718544). Necessary for hepatitis C virus replication in an otherwise non-permissive cell line (PubMed:11602742)
Protein Sequence
10
MVQAWYMDDA
20
PGDPRQPHRP
30
DPGRPVGLEQ
40
LRRLGVLYWK
50
LDADKYENDP
60
ELEKIRRERN
70
YSWMDIITIC
80
KDKLPNYEEK
90
IKMFYEEHLH
100
LDDEIRYILD
110
GSGYFDVRDK
120
EDQWIRIFME
130
KGDMVTLPAG
140
IYHRFTVDEK
150
NYTKAMRLFV
160
GEPVWTAYNR
170
PADHFEARGQ
YVKFLAQTA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0010308 |
acireductone dioxygenase (Ni2+-requiring) activity |
| Molecular Function |
GO:0010309 |
acireductone dioxygenase [iron(II)-requiring] activity |
| Molecular Function |
GO:0005506 |
iron ion binding |
| Molecular Function |
GO:0016151 |
nickel cation binding |
| Molecular Function |
GO:0016491 |
oxidoreductase activity |
| Biological Process |
GO:0019509 |
L-methionine salvage from methylthioadenosine |
| Biological Process |
GO:0006555 |
methionine metabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.