Search Results

Overview

Uniprot ID	Q9C0C9
Protein Name	(E3-independent) E2 ubiquitin-conjugating enzyme
Gene Name	UBE2O
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
521	KSIPLSIKNLKRKHK

Function

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). Mediates the monoubiquitination of cytoplasmic CTNNA1 to promote cell-to-extracellular matrix adhesions. Mechanistically, ubiquitination of CTNNA1 triggers a binding switch from beta- catenin/CTNNB1 to vinculin/VCL that directs CTNNA1 to mediate matrix adhesion (PubMed:40983751)

Protein Sequence

10 MADPAAPTPA 20 APAPAQAPAP 30 APEAVPAPAA 40 APVPAPAPAS 50 DSASGPSSDS 60 GPEAGSQRLL 70 FSHDLVSGRY 80 RGSVHFGLVR 90 LIHGEDSDSE 100 GEEEGRGSSG 110 CSEAGGAGHE 120 EGRASPLRRG 130 YVRVQWYPEG 140 VKQHVKETKL 150 KLEDRSVVPR 160 DVVRHMRSTD 170 SQCGTVIDVN 180 IDCAVKLIGT 190 NCIIYPVNSK 200 DLQHIWPFMY 210 GDYIAYDCWL 220 GKVYDLKNQI 230 ILKLSNGARC 240 SMNTEDGAKL 250 YDVCPHVSDS 260 GLFFDDSYGF 270 YPGQVLIGPA 280 KIFSSVQWLS 290 GVKPVLSTKS 300 KFRVVVEEVQ 310 VVELKVTWIT 320 KSFCPGGTDS 330 VSPPPSVITQ 340 ENLGRVKRLG 350 CFDHAQRQLG 360 ERCLYVFPAK 370 VEPAKIAWEC 380 PEKNCAQGEG 390 SMAKKVKRLL 400 KKQVVRIMSC 410 SPDTQCSRDH 420 SMEDPDKKGE 430 SKTKSEAESA 440 SPEETPDGSA 450 SPVEMQDEGA 460 EEPHEAGEQL 470 PPFLLKEGRD 480 DRLHSAEQDA 490 DDEAADDTDD 500 TSSVTSSASS 510 TTSSQSGSGT 520 SRKKSIPLSI 530 KNLKRKHKRK 540 KNKITRDFKP 550 GDRVAVEVVT 560 TMTSADVMWQ 570 DGSVECNIRS 580 NDLFPVHHLD 590 NNEFCPGDFV 600 VDKRVQSCPD 610 PAVYGVVQSG 620 DHIGRTCMVK 630 WFKLRPSGDD 640 VELIGEEEDV 650 SVYDIADHPD 660 FRFRTTDIVI 670 RIGNTEDGAP 680 HKEDEPSVGQ 690 VARVDVSSKV 700 EVVWADNSKT 710 IILPQHLYNI 720 ESEIEESDYD 730 SVEGSTSGAS 740 SDEWEDDSDS 750 WETDNGLVED 760 EHPKIEEPPI 770 PPLEQPVAPE 780 DKGVVISEEA 790 ATAAVQGAVA 800 MAAPMAGLME 810 KAGKDGPPKS 820 FRELKEAIKI 830 LESLKNMTVE 840 QLLTGSPTSP 850 TVEPEKPTRE 860 KKFLDDIKKL 870 QENLKKTLDN 880 VAIVEEEKME 890 AVPDVERKED 900 KPEGQSPVKA 910 EWPSETPVLC 920 QQCGGKPGVT 930 FTSAKGEVFS 940 VLEFAPSNHS 950 FKKIEFQPPE 960 AKKFFSTVRK 970 EMALLATSLP 980 EGIMVKTFED 990 RMDLFSALIK 1000 GPTRTPYEDG 1010 LYLFDIQLPN 1020 IYPAVPPHFC 1030 YLSQCSGRLN 1040 PNLYDNGKVC 1050 VSLLGTWIGK 1060 GTERWTSKSS 1070 LLQVLISIQG 1080 LILVNEPYYN 1090 EAGFDSDRGL 1100 QEGYENSRCY 1110 NEMALIRVVQ 1120 SMTQLVRRPP 1130 EVFEQEIRQH 1140 FSTGGWRLVN 1150 RIESWLETHA 1160 LLEKAQALPN 1170 GVPKASSSPE 1180 PPAVAELSDS 1190 GQQEPEDGGP 1200 APGEASQGSD 1210 SEGGAQGLAS 1220 ASRDHTDQTS 1230 ETAPDASVPP 1240 SVKPKKRRKS 1250 YRSFLPEKSG 1260 YPDIGFPLFP 1270 LSKGFIKSIR 1280 GVLTQFRAAL 1290 LEAGMPECTE DK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016604	nuclear body
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0061631	ubiquitin conjugating enzyme activity
Molecular Function	GO:0061630	ubiquitin protein ligase activity
Molecular Function	GO:0004842	ubiquitin-protein transferase activity
Biological Process	GO:0030513	positive regulation of BMP signaling pathway
Biological Process	GO:0070534	protein K63-linked ubiquitination
Biological Process	GO:0006513	protein monoubiquitination
Biological Process	GO:0042147	retrograde transport, endosome to Golgi

Reference

[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.