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Overview

Uniprot IDQ9CQR4
Protein NameAcyl-coenzyme A thioesterase 13
Gene NameAcot13
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
127 LTNKTTGKLIAQGRH
13 QNLREVMKVMFKVPG
17 EVMKVMFKVPGFDRV
27 GFDRVLEKVTLVSAA
37 LVSAAPEKLICEMKV
43 EKLICEMKVEEQHTN

Function

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:19405909). Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (PubMed:19405909). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity). May play a role in controlling adaptive thermogenesis (PubMed:24072708)

Protein Sequence

10 MSSMTQNLRE 20 VMKVMFKVPG 30 FDRVLEKVTL 40 VSAAPEKLIC 50 EMKVEEQHTN 60 KLGTLHGGLT 70 ATLVDSISTM 80 ALMCTERGAP 90 GVSVDMNITY 100 MSPAKIGEEI 110 VITAHILKQG 120 KTLAFASVDL 130 TNKTTGKLIA 140 QGRHTKHLGN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005819 spindle
Molecular Function GO:0047617 fatty acyl-CoA hydrolase activity
Biological Process GO:0006629 lipid metabolic process
Biological Process GO:0120163 negative regulation of cold-induced thermogenesis
Biological Process GO:0051289 protein homotetramerization

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.