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Overview

Uniprot IDQ9D8B3
Protein NameCharged multivesicular body protein 4b
Gene NameChmp4b
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
17 AGGGKAGKGGPTPQE
6 **MSVFGKLFGAGGG

Function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan. Majority of the protein exists in a folded closed conformation (By similarity)

Protein Sequence

10 MSVFGKLFGA 20 GGGKAGKGGP 30 TPQEAIQRLR 40 DTEEMLSKKQ 50 EFLEKKIEQE 60 LTAAKKHGTK 70 NKRAALQALK 80 RKKRYEKQLA 90 QIDGTLSTIE 100 FQREALENAN 110 TNTEVLKNMG 120 YAAKAMKAAH 130 DNMDIDKVDE 140 LMQDIADQQE 150 LAEEISTAIS 160 KPVGFGEEFD 170 EDELMAELEE 180 LEQEELDKNL 190 LEISGPETVP 200 LPNVPSVALP 210 SKPAKKKEEE 220 DDDMKELENW AGSM

Gene Ontology

Classification GO ID Description
Cellular Component GO:1904930 amphisome membrane
Cellular Component GO:0000421 autophagosome membrane
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0009898 cytoplasmic side of plasma membrane
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005768 endosome
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0000815 ESCRT III complex
Cellular Component GO:0098978 glutamatergic synapse
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0005828 kinetochore microtubule
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0016020 membrane
Cellular Component GO:0030117 membrane coat
Cellular Component GO:0030496 midbody
Cellular Component GO:0005771 multivesicular body
Cellular Component GO:0032585 multivesicular body membrane
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0005643 nuclear pore
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0014069 postsynaptic density
Cellular Component GO:0031982 vesicle
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0042803 protein homodimerization activity
Biological Process GO:0010458 exit from mitosis
Biological Process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
Biological Process GO:0090148 membrane fission
Biological Process GO:0000281 mitotic cytokinesis
Biological Process GO:0036258 multivesicular body assembly
Biological Process GO:0061763 multivesicular body-lysosome fusion
Biological Process GO:0031468 nuclear membrane reassembly
Biological Process GO:0015031 protein transport
Biological Process GO:0010506 regulation of autophagy
Biological Process GO:0006900 vesicle budding from membrane
Biological Process GO:0051469 vesicle fusion with vacuole

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.