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Overview

Uniprot IDQ9DCN2
Protein NameNADH-cytochrome b5 reductase 3
Gene NameCyb5r3
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
164 FAIRADKKSNPVVRT

Function

Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor

Protein Sequence

10 MGAQLSTLSH 20 VVLSPVWFIY 30 SLFMKLFQRS 40 TPAITLENPD 50 IKYPLRLIDK 60 EVISPDTRRF 70 RFALPSPQHI 80 LGLPIGQHIY 90 LSTRIDGNLV 100 IRPYTPVSSD 110 DDKGFVDLVV 120 KVYFKDTHPK 130 FPAGGKMSQY 140 LENMKIGDTI 150 EFRGPNGLLV 160 YQGKGKFAIR 170 ADKKSNPVVR 180 TVKSVGMIAG 190 GTGITPMLQV 200 IRAVLKDPND 210 HTVCYLLFAN 220 QSEKDILLRP 230 ELEELRNEHS 240 ARFKLWYTVD 250 KAPDAWDYSQ 260 GFVNEEMIRD 270 HLPTPGEEPL 280 ILMCGPPPMI 290 QFACLPNLER 300 VGHPKERCFT F

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0005811 lipid droplet
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:1903958 nitric-oxide synthase complex
Cellular Component GO:0033011 perinuclear theca
Cellular Component GO:0120238 sperm glycocalyx
Cellular Component GO:0120212 sperm head-tail coupling apparatus
Cellular Component GO:0097225 sperm midpiece
Cellular Component GO:0097228 sperm principal piece
Molecular Function GO:0043531 ADP binding
Molecular Function GO:0016208 AMP binding
Molecular Function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
Molecular Function GO:0090524 cytochrome-b5 reductase activity, acting on NADH
Molecular Function GO:0071949 FAD binding
Molecular Function GO:0050660 flavin adenine dinucleotide binding
Molecular Function GO:0051287 NAD binding
Molecular Function GO:0050421 nitrite reductase (NO-forming) activity
Biological Process GO:0006695 cholesterol biosynthetic process
Biological Process GO:0006809 nitric oxide biosynthetic process

Reference

[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.