Overview
| Uniprot ID | Q9EQU5 |
|---|
| Protein Name | Protein SET |
|---|
| Gene Name | Set |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 23 |
PRPAAAPKLEDKSAS |
| 27 |
AAPKLEDKSASPGLP |
Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher (By similarity)
Protein Sequence
10
MAPKRQSAIL
20
PQPKKPRPAA
30
APKLEDKSAS
40
PGLPKGEKEQ
50
QEAIEHIDEV
60
QNEIDRLNEQ
70
ASEEILKVEQ
80
KYNKLRQPFF
90
QKRSELIAKI
100
PNFWVTTFVN
110
HPQVSALLGE
120
EDEEALHYLT
130
RVEVTEFEDI
140
KSGYRIDFYF
150
DENPYFENKV
160
LSKEFHLNES
170
GDPSSKSTEI
180
KWKSGKDLTK
190
RSSQTQNKAS
200
RKRQHEEPES
210
FFTWFTDHSD
220
AGADELGEVI
230
KDDIWPNPLQ
240
YYLVPDMDDE
250
EGEAEDDDDD
260
DEEEEGLEDI
270
DEEGDEDEGE
280
EDDDEDEGEE
GEEDEGEDD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0042393 |
histone binding |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0043524 |
negative regulation of neuron apoptotic process |
| Biological Process |
GO:0006334 |
nucleosome assembly |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.