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Overview

Uniprot IDQ9GLV6
Protein NameAntiviral innate immune response receptor RIG-I
Gene NameRIGI
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
638 TRTILFVKTRALVDA
647 RALVDALKKWIKENP
782 DEAVFKEKIHQIQIR
864 GNIEKRAKIYCARPD
99 WDFQKIEKLEEYRSL

Function

Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines. Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments. The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms. Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons. Ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae, Rhabdoviridae: vesicular stomatitis virus (VSV) Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome. Detects dsRNA produced from non-self dsDNA by RNA polymerase III. May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration

Protein Sequence

10 MTAEQRRNLH 20 AFGDYVRKTL 30 DPTFILSYMA 40 PWFRDDEVQH 50 IQAEKNNKGP 60 TEAASLFLQF 70 LLELQEEGWF 80 RGFLDALNQA 90 GYCGLCEAIE 100 SWDFQKIEKL 110 EEYRSLLRRL 120 QPEFKTTINP 130 KDILPEIAEC 140 LISQECEEIL 150 QICSSKGLMA 160 GAEKMVECLL 170 RSDKENWPKT 180 LKLALEKEES 190 RFSELWMVDK 200 GAEDVKMKDL 210 EDDEMKTCDV 220 QIFYKEEPEN 230 QNLSQNSCSS 240 SAPHTYSPLK 250 PRKYQLELAL 260 PAQNGKNTII 270 CAPTGCGKTF 280 VSLLICEHHL 290 KKFPRGRKGK 300 VVFFAIQLPV 310 YEQQKSVFSK 320 HFERLGYKVA 330 GISGATSDTV 340 CVEQIVENSD 350 IIILTPQILV 360 NCLTNGTIPS 370 LSVFTLMIFD 380 ECHNTSKQHP 390 YNVIMFSYLD 400 RKLGGSSDSL 410 PQVIGLTASV 420 GVGDAKNKAE 430 ATEYICKLCA 440 SLDTSVIATV 450 RDNLEELEEV 460 VYKPQKFFRK 470 VELRTTDRFK 480 CIISQLMMEI 490 ESLAKSIFEE 500 LGTITLGGLF 510 QIQNSNFGTQ 520 KYEQWIVKVQ 530 KECAVFQMPD 540 KDKESRICKA 550 LFSYMSHLRI 560 YNDALIINEH 570 ARMKDALDYL 580 KDFFRNIRAA 590 GFDEIEQDLT 600 QRFEEKLQEL 610 ESISIDPSNE 620 NPKLRDLCFI 630 LQEEYHLNPE 640 TRTILFVKTR 650 ALVDALKKWI 660 KENPKLSFLK 670 PSILTGRGKT 680 NQNIGMTLPA 690 QKCVLDTFRT 700 DKDNKILITT 710 SVADEGIDIA 720 QCNLVILYEY 730 VGNVIKMIQT 740 RGRGRARGSK 750 CFLLTANADL 760 IDKEKMNMYK 770 EEMMNGAILI 780 LQTWDEAVFK 790 EKIHQIQIRE 800 KIIRDNQGKP 810 EPVPDKKTKK 820 LLCKKCKAFA 830 CYTADIRMVE 840 KCHFTVVGDA 850 FRERFVSKLH 860 PKPKSFGNIE 870 KRAKIYCARP 880 DCSHDWGIYV 890 RYKAFEMPFI 900 KIESFVVEDI 910 ATGVQTVHAK 920 WKDFNFEKLS 930 FDAAEMAGGA 940 QDLGLQGMGN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005923 bicellular tight junction
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:1990904 ribonucleoprotein complex
Cellular Component GO:0032587 ruffle membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003725 double-stranded RNA binding
Molecular Function GO:0003724 RNA helicase activity
Molecular Function GO:0003727 single-stranded RNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0140374 antiviral innate immune response
Biological Process GO:0071360 cellular response to exogenous dsRNA
Biological Process GO:0002753 cytoplasmic pattern recognition receptor signaling pathway
Biological Process GO:0045087 innate immune response
Biological Process GO:0002230 positive regulation of defense response to virus by host
Biological Process GO:0032727 positive regulation of interferon-alpha production
Biological Process GO:0032728 positive regulation of interferon-beta production
Biological Process GO:0032755 positive regulation of interleukin-6 production
Biological Process GO:0002735 positive regulation of myeloid dendritic cell cytokine production
Biological Process GO:0060760 positive regulation of response to cytokine stimulus
Biological Process GO:0032760 positive regulation of tumor necrosis factor production
Biological Process GO:0030334 regulation of cell migration
Biological Process GO:0043330 response to exogenous dsRNA
Biological Process GO:0039529 RIG-I signaling pathway

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.