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Overview

Uniprot IDQ9GMB0
Protein NameDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Gene NameRPN1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
188 RNVESYTKLGNPTRS
517 VSTLNSGKKSLELEH
518 STLNSGKKSLELEHK
525 KSLELEHKALTSEVA
539 ALLQSRLKTEGSDLC
560 QKLDAQVKELVLKSA
565 QVKELVLKSAVEAER
580 LVAGKLKKDTYIENE
588 DTYIENEKLISGKRQ

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (Probable). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity)

Protein Sequence

10 MEAPAVCLLP 20 LLLLLWAWAP 30 APGRASPEAL 40 PLVNEDVKRT 50 VDLSSHLAKV 60 TAEVVLAHAG 70 SSSSPRAASF 80 LLALEPELEA 90 RLAHLGVQVK 100 GEDEEENNLE 110 VRETKIKGKR 120 GRFFTVTLPV 130 ALDPGAKISV 140 TVETVYTHVL 150 QPYPTQITQS 160 EKQFVVFEGN 170 HYFYSPYPTK 180 SQSMRVKLAS 190 RNVESYTKLG 200 NPTRSEDLLD 210 YGPFRDVPPY 220 SQDTFKVHSE 230 NNSPFLTITS 240 MTRVIEVSHW 250 GNIAVEENVD 260 LKHTGAVLKG 270 PFSRYDYQRQ 280 PDSGISSIRS 290 FKTILPAAAQ 300 DVYYRDEIGN 310 VSTSHLLILD 320 DSVEMEIRPR 330 FPLFGGWKTH 340 YIVGYNLPSY 350 EYLYNLGDQY 360 ALKMRLVDHV 370 FDEQVIDSLT 380 VKIILPEGAK 390 NIQVDSPYEI 400 SRAPDELHYT 410 YLDTFGRPVI 420 VAHKKNLVEQ 430 HIQDIVVHYT 440 FNKVLMLQEP 450 LLVVAAFYIL 460 FFTVIVYVRL 470 DFSITKDPAA 480 EARMKVACIT 490 EQVLTLVNKR 500 IGLYRHFDET 510 INRYKQSRDV 520 STLNSGKKSL 530 ELEHKALTSE 540 VALLQSRLKT 550 EGSDLCDKVS 560 EMQKLDAQVK 570 ELVLKSAVEA 580 ERLVAGKLKK 590 DTYIENEKLI 600 SGKRQELVTK IDHILDAL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0008250 oligosaccharyltransferase complex
Biological Process GO:0009101 glycoprotein biosynthetic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.