Overview
| Uniprot ID | Q9GZQ8 |
|---|
| Protein Name | Microtubule-associated protein 1 light chain 3 beta |
|---|
| Gene Name | MAP1LC3B |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 30 |
IREQHPTKIPVIIER |
| 42 |
IERYKGEKQLPVLDK |
Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355)
Protein Sequence
10
MPSEKTFKQR
20
RTFEQRVEDV
30
RLIREQHPTK
40
IPVIIERYKG
50
EKQLPVLDKT
60
KFLVPDHVNM
70
SELIKIIRRR
80
LQLNANQAFF
90
LLVNGHSMVS
100
VSTPISEVYE
110
SEKDEDGFLY
120
MVYASQETFG
MKLSV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005776 |
autophagosome |
| Cellular Component |
GO:0000421 |
autophagosome membrane |
| Cellular Component |
GO:0005930 |
axoneme |
| Cellular Component |
GO:0031410 |
cytoplasmic vesicle |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0012505 |
endomembrane system |
| Cellular Component |
GO:0005874 |
microtubule |
| Cellular Component |
GO:0031966 |
mitochondrial membrane |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0031090 |
organelle membrane |
| Molecular Function |
GO:0097001 |
ceramide binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0008429 |
phosphatidylethanolamine binding |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Biological Process |
GO:0000045 |
autophagosome assembly |
| Biological Process |
GO:0097352 |
autophagosome maturation |
| Biological Process |
GO:0006914 |
autophagy |
| Biological Process |
GO:0006995 |
cellular response to nitrogen starvation |
| Biological Process |
GO:0009267 |
cellular response to starvation |
| Biological Process |
GO:0016236 |
macroautophagy |
| Biological Process |
GO:0000423 |
mitophagy |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.