Overview
| Uniprot ID | Q9H0C2 |
|---|
| Protein Name | ADP/ATP translocase 4 |
|---|
| Gene Name | SLC25A31 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 35 |
GVAAAVSKTAVAPIE |
Function
ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity) (PubMed:15670820). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). Specifically required during spermatogenesis, probably to mediate ADP:ATP exchange in spermatocytes (PubMed:17137571). Large ATP supplies from mitochondria may be critical for normal progression of spermatogenesis during early stages of meiotic prophase I, including DNA double-strand break repair and chromosomal synapsis (By similarity). In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (By similarity). Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A31/ANT4 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Among nucleotides, may also exchange ADP for dATP and dADP (PubMed:15670820). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (By similarity). It is however unclear if SLC25A31/ANT4 constitutes a pore-forming component of mPTP or regulates it (By similarity)
Protein Sequence
10
MHREPAKKKA
20
EKRLFDASSF
30
GKDLLAGGVA
40
AAVSKTAVAP
50
IERVKLLLQV
60
QASSKQISPE
70
ARYKGMVDCL
80
VRIPREQGFF
90
SFWRGNLANV
100
IRYFPTQALN
110
FAFKDKYKQL
120
FMSGVNKEKQ
130
FWRWFLANLA
140
SGGAAGATSL
150
CVVYPLDFAR
160
TRLGVDIGKG
170
PEERQFKGLG
180
DCIMKIAKSD
190
GIAGLYQGFG
200
VSVQGIIVYR
210
ASYFGAYDTV
220
KGLLPKPKKT
230
PFLVSFFIAQ
240
VVTTCSGILS
250
YPFDTVRRRM
260
MMQSGEAKRQ
270
YKGTLDCFVK
280
IYQHEGISSF
290
FRGAFSNVLR
300
GTGGALVLVL
310
YDKIKEFFHI
DIGGR
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016020 |
membrane |
| Biological Process |
GO:0007283 |
spermatogenesis |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005757 |
mitochondrial permeability transition pore complex |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0005471 |
ATP:ADP antiporter activity |
| Biological Process |
GO:0030154 |
cell differentiation |
| Biological Process |
GO:0007141 |
male meiosis I |
| Biological Process |
GO:0140021 |
mitochondrial ADP transmembrane transport |
| Biological Process |
GO:1990544 |
mitochondrial ATP transmembrane transport |
| Biological Process |
GO:0046902 |
regulation of mitochondrial membrane permeability |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.