Overview
| Uniprot ID | Q9H0E7 |
|---|
| Protein Name | Ubiquitin carboxyl-terminal hydrolase 44 |
|---|
| Gene Name | USP44 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 550 |
KRRRFSSKPVVLTEA |
Function
Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) (PubMed:17443180). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C (PubMed:17443180). Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint (PubMed:17443180). Also promotes the deubiquitination of histone H2A and H2B (PubMed:23615962, PubMed:27880911). Recruited to RNF8/RNF168-ubiquitinated chromatin surrounding double stranded breaks (DSBs), promotes hydrolysis of such ubiquitin conjugates, thus negatively regulating protein recruitment to damaged chromatin (PubMed:23615962). Participates in nucleotide excision repair (NER) pathway by deubiquitinating DDB2 to prevent its premature degradation so it can remain on damaged chromatin (By similarity). Promotes FOXP3 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability and increased regulatory T-cell lineage stability (PubMed:32644293). Also plays a positive role in innate immune response to DNA viruses by deubiquitinating STING1, selectively removing its 'Lys-48'-linked polyubiquitin chains and stabilizing it (PubMed:31968013)
Protein Sequence
10
MLAMDTCKHV
20
GQLQLAQDHS
30
SLNPQKWHCV
40
DCNTTESIWA
50
CLSCSHVACG
60
RYIEEHALKH
70
FQESSHPVAL
80
EVNEMYVFCY
90
LCDDYVLNDN
100
TTGDLKLLRR
110
TLSAIKSQNY
120
HCTTRSGRFL
130
RSMGTGDDSY
140
FLHDGAQSLL
150
QSEDQLYTAL
160
WHRRRILMGK
170
IFRTWFEQSP
180
IGRKKQEEPF
190
QEKIVVKREV
200
KKRRQELEYQ
210
VKAELESMPP
220
RKSLRLQGLA
230
QSTIIEIVSV
240
QVPAQTPASP
250
AKDKVLSTSE
260
NEISQKVSDS
270
SVKRRPIVTP
280
GVTGLRNLGN
290
TCYMNSVLQV
300
LSHLLIFRQC
310
FLKLDLNQWL
320
AMTASEKTRS
330
CKHPPVTDTV
340
VYQMNECQEK
350
DTGFVCSRQS
360
SLSSGLSGGA
370
SKGRKMELIQ
380
PKEPTSQYIS
390
LCHELHTLFQ
400
VMWSGKWALV
410
SPFAMLHSVW
420
RLIPAFRGYA
430
QQDAQEFLCE
440
LLDKIQRELE
450
TTGTSLPALI
460
PTSQRKLIKQ
470
VLNVVNNIFH
480
GQLLSQVTCL
490
ACDNKSNTIE
500
PFWDLSLEFP
510
ERYQCSGKDI
520
ASQPCLVTEM
530
LAKFTETEAL
540
EGKIYVCDQC
550
NSKRRRFSSK
560
PVVLTEAQKQ
570
LMICHLPQVL
580
RLHLKRFRWS
590
GRNNREKIGV
600
HVGFEEILNM
610
EPYCCRETLK
620
SLRPECFIYD
630
LSAVVMHHGK
640
GFGSGHYTAY
650
CYNSEGGFWV
660
HCNDSKLSMC
670
TMDEVCKAQA
680
YILFYTQRVT
690
ENGHSKLLPP
700
ELLLGSQHPN
710
EDADTSSNEI
LS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0001650 |
fibrillar center |
| Cellular Component |
GO:0072686 |
mitotic spindle |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0004843 |
cysteine-type deubiquitinase activity |
| Molecular Function |
GO:0101005 |
deubiquitinase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0140374 |
antiviral innate immune response |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0007059 |
chromosome segregation |
| Biological Process |
GO:0045841 |
negative regulation of mitotic metaphase/anaphase transition |
| Biological Process |
GO:1904667 |
negative regulation of ubiquitin protein ligase activity |
| Biological Process |
GO:0006289 |
nucleotide-excision repair |
| Biological Process |
GO:0043161 |
proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0016579 |
protein deubiquitination |
| Biological Process |
GO:0010564 |
regulation of cell cycle process |
| Biological Process |
GO:0090266 |
regulation of mitotic cell cycle spindle assembly checkpoint |
| Biological Process |
GO:0045066 |
regulatory T cell differentiation |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.