Overview
| Uniprot ID | Q9H1D9 |
|---|
| Protein Name | DNA-directed RNA polymerase III subunit RPC6 |
|---|
| Gene Name | POLR3F |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 191 |
CFKFLQSKAETARES |
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:21358628, PubMed:33558764, PubMed:34675218). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state (PubMed:20413673, PubMed:33558764, PubMed:33558766). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370, PubMed:30211253). Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628)
Protein Sequence
10
MAEVKVKVQP
20
PDADPVEIEN
30
RIIELCHQFP
40
HGITDQVIQN
50
EMPHIEAQQR
60
AVAINRLLSM
70
GQLDLLRSNT
80
GLLYRIKDSQ
90
NAGKMKGSDN
100
QEKLVYQIIE
110
DAGNKGIWSR
120
DIRYKSNLPL
130
TEINKILKNL
140
ESKKLIKAVK
150
SVAASKKKVY
160
MLYNLQPDRS
170
VTGGAWYSDQ
180
DFESEFVEVL
190
NQQCFKFLQS
200
KAETARESKQ
210
NPMIQRNSSF
220
ASSHEVWKYI
230
CELGISKVEL
240
SMEDIETILN
250
TLIYDGKVEM
260
TIIAAKEGTV
270
GSVDGHMKLY
280
RAVNPIIPPT
290
GLVRAPCGLC
300
PVFDDCHEGG
310
EISPSNCIYM
TEWLEF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005666 |
RNA polymerase III complex |
| Molecular Function |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
| Molecular Function |
GO:0003899 |
DNA-directed RNA polymerase activity |
| Molecular Function |
GO:0003690 |
double-stranded DNA binding |
| Biological Process |
GO:0051607 |
defense response to virus |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0045089 |
positive regulation of innate immune response |
| Biological Process |
GO:0032728 |
positive regulation of interferon-beta production |
| Biological Process |
GO:0006359 |
regulation of transcription by RNA polymerase III |
| Biological Process |
GO:0006386 |
termination of RNA polymerase III transcription |
| Biological Process |
GO:0006383 |
transcription by RNA polymerase III |
| Biological Process |
GO:0006384 |
transcription initiation at RNA polymerase III promoter |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.