Search Results

Overview

Uniprot IDQ9H1E3
Protein NameNuclear ubiquitous casein and cyclin-dependent kinase substrate 1
Gene NameNUCKS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
102 AASKAASKQREMLME
171 RKEKKMPKPRLKATV
175 KMPKPRLKATVTPSP
184 TVTPSPVKGKGKVGR
186 TPSPVKGKGKVGRPT
188 SPVKGKGKVGRPTAS
196 VGRPTASKASKEKTP
201 ASKASKEKTPSPKEE
35 RDSGPPTKKIRSSPR
36 DSGPPTKKIRSSPRE
52 KNKRRSGKNSQEDSE
9 SRPVRNRKVVDYSQF
98 QQRQAASKAASKQRE

Function

Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR) (PubMed:26323318). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, but with less affinity than RAD51AP1 (PubMed:26323318)

Protein Sequence

10 MSRPVRNRKV 20 VDYSQFQESD 30 DADEDYGRDS 40 GPPTKKIRSS 50 PREAKNKRRS 60 GKNSQEDSED 70 SEDKDVKTKK 80 DDSHSAEDSE 90 DEKEDHKNVR 100 QQRQAASKAA 110 SKQREMLMED 120 VGSEEEQEEE 130 DEAPFQEKDS 140 GSDEDFLMED 150 DDDSDYGSSK 160 KKNKKMVKKS 170 KPERKEKKMP 180 KPRLKATVTP 190 SPVKGKGKVG 200 RPTASKASKE 210 KTPSPKEEDE 220 EPESPPEKKT 230 STSPPPEKSG 240 DEGSEDEAPS GED

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0140297 DNA-binding transcription factor binding
Molecular Function GO:0003690 double-stranded DNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003697 single-stranded DNA binding
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0071481 cellular response to X-ray
Biological Process GO:0006325 chromatin organization
Biological Process GO:0000724 double-strand break repair via homologous recombination
Biological Process GO:0035822 gene conversion
Biological Process GO:0044829 host-mediated activation of viral genome replication
Biological Process GO:0043923 host-mediated activation of viral transcription
Biological Process GO:0044827 host-mediated perturbation of viral genome replication
Biological Process GO:0036297 interstrand cross-link repair
Biological Process GO:0001678 intracellular glucose homeostasis
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0006275 regulation of DNA replication
Biological Process GO:0060382 regulation of DNA strand elongation
Biological Process GO:0046626 regulation of insulin receptor signaling pathway
Biological Process GO:0031297 replication fork processing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[6] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[7] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[8] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[9] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[10] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[11] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[12] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.