Search Results

Overview

Uniprot ID	Q9H1J1
Protein Name	Regulator of nonsense transcripts 3A
Gene Name	UPF3A
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
25	RGPSGREKLSALEVQ
461	GGNRRICKAEGSGTG

Function

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation

Protein Sequence

10 MRSEKEGAGG 20 LRAAVAARGP 30 SGREKLSALE 40 VQFHRDSQQQ 50 EAETPPTSSS 60 GCGGGAGKPR 70 EEKRTALSKV 80 VIRRLPPGLT 90 KEQLEEQLRP 100 LPAHDYFEFF 110 AADLSLYPHL 120 YSRAYINFRN 130 PDDILLFRDR 140 FDGYIFLDSK 150 GLEYPAVVEF 160 APFQKIAKKK 170 LRKKDAKTGS 180 IEDDPEYKKF 190 LETYCVEEEK 200 TSANPETLLG 210 EMEAKTRELI 220 ARRTTPLLEY 230 IKNRKLEKQR 240 IREEKREERR 250 RRELEKKRLR 260 EEEKRRRREE 270 ERCKKKETDK 280 QKKIAEKEVR 290 IKLLKKPEKG 300 EEPTTEKPKE 310 RGEEIDTGGG 320 KQESCAPGAV 330 VKARPMEGSL 340 EEPQETSHSG 350 SDKEHRDVER 360 SQEQESEAQR 370 YHVDDGRRHR 380 AHHEPERLSR 390 RSEDEQRWGK 400 GPGQDRGKKG 410 SQDSGAPGEA 420 MERLGRAQRC 430 DDSPAPRKER 440 LANKDRPALQ 450 LYDPGARFRA 460 RECGGNRRIC 470 KAEGSGTGPE KREEAE

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0035145	exon-exon junction complex
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0003729	mRNA binding
Molecular Function	GO:0140311	protein sequestering activity
Molecular Function	GO:0042162	telomeric DNA binding
Biological Process	GO:0051028	mRNA transport
Biological Process	GO:2000623	negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Biological Process	GO:0045727	positive regulation of translation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.