Overview
| Uniprot ID | Q9H204 |
|---|
| Protein Name | Mediator of RNA polymerase II transcription subunit 28 |
|---|
| Gene Name | MED28 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 125 |
LRNELQRKDALVQKH |
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways
Protein Sequence
10
MAAPLGGMFS
20
GQPPGPPQAP
30
PGLPGQASLL
40
QAAPGAPRPS
50
SSTLVDELES
60
SFEACFASLV
70
SQDYVNGTDQ
80
EEIRTGVDQC
90
IQKFLDIARQ
100
TECFFLQKRL
110
QLSVQKPEQV
120
IKEDVSELRN
130
ELQRKDALVQ
140
KHLTKLRHWQ
150
QVLEDINVQH
160
KKPADIPQGS
170
LAYLEQASAN
IPAPLKPT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030864 |
cortical actin cytoskeleton |
| Cellular Component |
GO:0016592 |
mediator complex |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003779 |
actin binding |
| Biological Process |
GO:0032968 |
positive regulation of transcription elongation by RNA polymerase II |
| Biological Process |
GO:0060261 |
positive regulation of transcription initiation by RNA polymerase II |
| Biological Process |
GO:0051123 |
RNA polymerase II preinitiation complex assembly |
Reference
[1] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.