Search Results

Overview

Uniprot ID	Q9H223
Protein Name	EH domain-containing protein 4
Gene Name	EHD4
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
223	KIRVVLNKADQVDTQ
35	LRSLYLRKVLPLEEA
378	FTKFHSLKPKLIEAV

Function

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport (PubMed:17233914, PubMed:18331452). During sprouting angiogenesis, in complex with PACSIN2 and MICALL1, forms recycling endosome-like tubular structure at asymmetric adherens junctions to control CDH5 trafficking (By similarity)

Protein Sequence

10 MFSWMGRQAG 20 GRERAGGADA 30 VQTVTGGLRS 40 LYLRKVLPLE 50 EAYRFHEFHS 60 PALEDADFEN 70 KPMILLVGQY 80 STGKTTFIRY 90 LLEQDFPGMR 100 IGPEPTTDSF 110 IAVMYGETEG 120 STPGNALVVD 130 PKKPFRKLSR 140 FGNAFLNRFM 150 CSQLPNQVLK 160 SISVIDSPGI 170 LSGEKQRISR 180 GYDFCQVLQW 190 FAERVDRIIL 200 LFDAHKLDIS 210 DEFSEAIKAF 220 RGQDDKIRVV 230 LNKADQVDTQ 240 QLMRVYGALM 250 WSLGKVINTP 260 EVLRVYIGSF 270 WAQPLQNTDN 280 RRLFEAEAQD 290 LFRDIQSLPQ 300 KAAVRKLNDL 310 IKRARLAKVH 320 AYIISYLKKE 330 MPSVFGKENK 340 KRELISRLPE 350 IYIQLQREYQ 360 ISAGDFPEVK 370 AMQEQLENYD 380 FTKFHSLKPK 390 LIEAVDNMLS 400 NKISPLMNLI 410 SQEETSTPTQ 420 LVQGGAFDGT 430 TEGPFNQGYG 440 EGAKEGADEE 450 EWVVAKDKPV 460 YDELFYTLSP 470 INGKISGVNA 480 KKEMVTSKLP 490 NSVLGKIWKL 500 ADCDCDGMLD 510 EEEFALAKHL 520 IKIKLDGYEL 530 PSSLPPHLVP 540 PSHRKSLPKA D

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005912	adherens junction
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005769	early endosome
Cellular Component	GO:0031901	early endosome membrane
Cellular Component	GO:0030139	endocytic vesicle
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0055038	recycling endosome membrane
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0045296	cadherin binding
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0003676	nucleic acid binding
Molecular Function	GO:0030674	protein-macromolecule adaptor activity
Biological Process	GO:0002042	cell migration involved in sprouting angiogenesis
Biological Process	GO:0071363	cellular response to growth factor stimulus
Biological Process	GO:0060271	cilium assembly
Biological Process	GO:0032456	endocytic recycling
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0006907	pinocytosis
Biological Process	GO:0051260	protein homooligomerization
Biological Process	GO:0072659	protein localization to plasma membrane
Biological Process	GO:0030100	regulation of endocytosis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.