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Overview

Uniprot IDQ9H267
Protein NameVacuolar protein sorting-associated protein 33B
Gene NameVPS33B
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
461 AVESKVSKLVTDKAA
466 VSKLVTDKAAGKITD
470 VTDKAAGKITDAFSS

Function

May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen (PubMed:28017832). Mediates phagolysosomal fusion in macrophages (PubMed:18474358). Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity)

Protein Sequence

10 MAFPHRPDAP 20 ELPDFSMLKR 30 LARDQLIYLL 40 EQLPGKKDLF 50 IEADLMSPLD 60 RIANVSILKQ 70 HEVDKLYKVE 80 NKPALSSNEQ 90 LCFLVRPRIK 100 NMRYIASLVN 110 ADKLAGRTRK 120 YKVIFSPQKF 130 YACEMVLEEE 140 GIYGDVSCDE 150 WAFSLLPLDV 160 DLLSMELPEF 170 FRDYFLEGDQ 180 RWINTVAQAL 190 HLLSTLYGPF 200 PNCYGIGRCA 210 KMAYELWRNL 220 EEEEDGETKG 230 RRPEIGHIFL 240 LDRDVDFVTA 250 LCSQVVYEGL 260 VDDTFRIKCG 270 SVDFGPEVTS 280 SDKSLKVLLN 290 AEDKVFNEIR 300 NEHFSNVFGF 310 LSQKARNLQA 320 QYDRRRGMDI 330 KQMKNFVSQE 340 LKGLKQEHRL 350 LSLHIGACES 360 IMKKKTKQDF 370 QELIKTEHAL 380 LEGFNIREST 390 SYIEEHIDRQ 400 VSPIESLRLM 410 CLLSITENGL 420 IPKDYRSLKT 430 QYLQSYGPEH 440 LLTFSNLRRA 450 GLLTEQAPGD 460 TLTAVESKVS 470 KLVTDKAAGK 480 ITDAFSSLAK 490 RSNFRAISKK 500 LNLIPRVDGE 510 YDLKVPRDMA 520 YVFGGAYVPL 530 SCRIIEQVLE 540 RRSWQGLDEV 550 VRLLNCSDFA 560 FTDMTKEDKA 570 SSESLRLILV 580 VFLGGCTFSE 590 ISALRFLGRE 600 KGYRFIFLTT 610 AVTNSARLME AMSEVKA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030136 clathrin-coated vesicle
Biological Process GO:0017185 peptidyl-lysine hydroxylation
Biological Process GO:0090385 phagosome-lysosome fusion
Biological Process GO:0070889 platelet alpha granule organization
Biological Process GO:0015031 protein transport
Biological Process GO:0090330 regulation of platelet aggregation
Biological Process GO:0043589 skin morphogenesis
Biological Process GO:0016192 vesicle-mediated transport
Cellular Component GO:0033263 CORVET complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0031901 early endosome membrane
Cellular Component GO:0005768 endosome
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0030897 HOPS complex
Cellular Component GO:0005770 late endosome
Cellular Component GO:0031902 late endosome membrane
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005764 lysosome
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0031091 platelet alpha granule
Cellular Component GO:0055037 recycling endosome
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0032963 collagen metabolic process
Biological Process GO:0007032 endosome organization
Biological Process GO:0006886 intracellular protein transport
Biological Process GO:0032418 lysosome localization
Biological Process GO:0035855 megakaryocyte development
Biological Process GO:0032400 melanosome localization
Biological Process GO:0061025 membrane fusion

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.