Overview
| Uniprot ID | Q9H270 |
|---|
| Protein Name | Vacuolar protein sorting-associated protein 11 homolog |
|---|
| Gene Name | VPS11 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 295 |
RDRKVSPKSEFTSRD |
Function
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203). Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (PubMed:21148287). Involved in the retrograde Shiga toxin transport (PubMed:23593995)
Protein Sequence
10
MAAYLQWRRF
20
VFFDKELVKE
30
PLSNDGAAPG
40
ATPASGSAAS
50
KFLCLPPGIT
60
VCDSGRGSLV
70
FGDMEGQIWF
80
LPRSLQLTGF
90
QAYKLRVTHL
100
YQLKQHNILA
110
SVGEDEEGIN
120
PLVKIWNLEK
130
RDGGNPLCTR
140
IFPAIPGTEP
150
TVVSCLTVHE
160
NLNFMAIGFT
170
DGSVTLNKGD
180
ITRDRHSKTQ
190
ILHKGNYPVT
200
GLAFRQAGKT
210
THLFVVTTEN
220
VQSYIVSGKD
230
YPRVELDTHG
240
CGLRCSALSD
250
PSQDLQFIVA
260
GDECVYLYQP
270
DERGPCFAFE
280
GHKLIAHWFR
290
GYLIIVSRDR
300
KVSPKSEFTS
310
RDSQSSDKQI
320
LNIYDLCNKF
330
IAYSTVFEDV
340
VDVLAEWGSL
350
YVLTRDGRVH
360
ALQEKDTQTK
370
LEMLFKKNLF
380
EMAINLAKSQ
390
HLDSDGLAQI
400
FMQYGDHLYS
410
KGNHDGAVQQ
420
YIRTIGKLEP
430
SYVIRKFLDA
440
QRIHNLTAYL
450
QTLHRQSLAN
460
ADHTTLLLNC
470
YTKLKDSSKL
480
EEFIKKKSES
490
EVHFDVETAI
500
KVLRQAGYYS
510
HALYLAENHA
520
HHEWYLKIQL
530
EDIKNYQEAL
540
RYIGKLPFEQ
550
AESNMKRYGK
560
ILMHHIPEQT
570
TQLLKGLCTD
580
YRPSLEGRSD
590
REAPGCRANS
600
EEFIPIFANN
610
PRELKAFLEH
620
MSEVQPDSPQ
630
GIYDTLLELR
640
LQNWAHEKDP
650
QVKEKLHAEA
660
ISLLKSGRFC
670
DVFDKALVLC
680
QMHDFQDGVL
690
YLYEQGKLFQ
700
QIMHYHMQHE
710
QYRQVISVCE
720
RHGEQDPSLW
730
EQALSYFARK
740
EEDCKEYVAA
750
VLKHIENKNL
760
MPPLLVVQTL
770
AHNSTATLSV
780
IRDYLVQKLQ
790
KQSQQIAQDE
800
LRVRRYREET
810
TRIRQEIQEL
820
KASPKIFQKT
830
KCSICNSALE
840
LPSVHFLCGH
850
SFHQHCFESY
860
SESDADCPTC
870
LPENRKVMDM
880
IRAQEQKRDL
890
HDQFQHQLKC
900
SNDSFSVIAD
910
YFGRGVFNKL
920
TLLTDPPTAR
930
LTSSLEAGLQ
940
RDLLMHSRRG
T
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005776 |
autophagosome |
| Cellular Component |
GO:0030136 |
clathrin-coated vesicle |
| Cellular Component |
GO:0005769 |
early endosome |
| Cellular Component |
GO:0030139 |
endocytic vesicle |
| Cellular Component |
GO:0005768 |
endosome |
| Cellular Component |
GO:0010008 |
endosome membrane |
| Cellular Component |
GO:0030897 |
HOPS complex |
| Cellular Component |
GO:0005770 |
late endosome |
| Cellular Component |
GO:0031902 |
late endosome membrane |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0005764 |
lysosome |
| Molecular Function |
GO:0000166 |
nucleotide binding |
| Molecular Function |
GO:0019904 |
protein domain specific binding |
| Molecular Function |
GO:0030674 |
protein-macromolecule adaptor activity |
| Molecular Function |
GO:0019905 |
syntaxin binding |
| Molecular Function |
GO:0061630 |
ubiquitin protein ligase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0061909 |
autophagosome-lysosome fusion |
| Biological Process |
GO:0034058 |
endosomal vesicle fusion |
| Biological Process |
GO:0007032 |
endosome organization |
| Biological Process |
GO:0008333 |
endosome to lysosome transport |
| Biological Process |
GO:0006886 |
intracellular protein transport |
| Biological Process |
GO:0033147 |
negative regulation of intracellular estrogen receptor signaling pathway |
| Biological Process |
GO:0048284 |
organelle fusion |
| Biological Process |
GO:2000643 |
positive regulation of early endosome to late endosome transport |
| Biological Process |
GO:0045732 |
positive regulation of protein catabolic process |
| Biological Process |
GO:1903955 |
positive regulation of protein targeting to mitochondrion |
| Biological Process |
GO:0016567 |
protein ubiquitination |
| Biological Process |
GO:1902115 |
regulation of organelle assembly |
| Biological Process |
GO:0031647 |
regulation of protein stability |
| Biological Process |
GO:0035542 |
regulation of SNARE complex assembly |
| Biological Process |
GO:0007033 |
vacuole organization |
| Biological Process |
GO:0006904 |
vesicle docking involved in exocytosis |
Reference
[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.