Search Results

Overview

Uniprot ID	Q9H2U1
Protein Name	ATP-dependent DNA/RNA helicase DHX36
Gene Name	DHX36
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
139	NTPCSENKLDIQEKK
146	KLDIQEKKLINQEKK
152	KKLINQEKKMFRIRN
68	EIGMWYAKKQGQKNK
69	IGMWYAKKQGQKNKE

Function

Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:16150737, PubMed:18854321, PubMed:20472641, PubMed:21586581). Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed:14731398, PubMed:18279852, PubMed:21993297, PubMed:22238380, PubMed:25579584). G4 structures correspond to helical structures containing guanine tetrads (By similarity). Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-DNA and G4-RNA) (PubMed:16150737, PubMed:18842585, PubMed:20472641, PubMed:21586581, PubMed:24369427, PubMed:26195789). Plays a role in genomic integrity (PubMed:22238380). Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (PubMed:20472641, PubMed:21149580, PubMed:21846770, PubMed:22238380, PubMed:24151078, PubMed:25579584). Plays a role in transcriptional regulation (PubMed:21586581, PubMed:21993297). Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (PubMed:21993297). Plays a role in post-transcriptional regulation (PubMed:27940037). Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (PubMed:27940037). Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity). Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity). Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (PubMed:24369427, PubMed:26489465). Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3'-UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (PubMed:24369427). Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3'-UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (PubMed:26489465). Also binds to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (PubMed:14731398, PubMed:18279852). Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (PubMed:14731398). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1 (By similarity). Required for early embryonic development and hematopoiesis. Involved in the regulation of cardioblast differentiation and proliferation during heart development. Involved in spermatogonia differentiation. May play a role in ossification (By similarity)

Protein Sequence

10 MSYDYHQNWG 20 RDGGPRSSGG 30 GYGGGPAGGH 40 GGNRGSGGGG 50 GGGGGGRGGR 60 GRHPGHLKGR 70 EIGMWYAKKQ 80 GQKNKEAERQ 90 ERAVVHMDER 100 REEQIVQLLN 110 SVQAKNDKES 120 EAQISWFAPE 130 DHGYGTEVST 140 KNTPCSENKL 150 DIQEKKLINQ 160 EKKMFRIRNR 170 SYIDRDSEYL 180 LQENEPDGTL 190 DQKLLEDLQK 200 KKNDLRYIEM 210 QHFREKLPSY 220 GMQKELVNLI 230 DNHQVTVISG 240 ETGCGKTTQV 250 TQFILDNYIE 260 RGKGSACRIV 270 CTQPRRISAI 280 SVAERVAAER 290 AESCGSGNST 300 GYQIRLQSRL 310 PRKQGSILYC 320 TTGIILQWLQ 330 SDPYLSSVSH 340 IVLDEIHERN 350 LQSDVLMTVV 360 KDLLNFRSDL 370 KVILMSATLN 380 AEKFSEYFGN 390 CPMIHIPGFT 400 FPVVEYLLED 410 VIEKIRYVPE 420 QKEHRSQFKR 430 GFMQGHVNRQ 440 EKEEKEAIYK 450 ERWPDYVREL 460 RRRYSASTVD 470 VIEMMEDDKV 480 DLNLIVALIR 490 YIVLEEEDGA 500 ILVFLPGWDN 510 ISTLHDLLMS 520 QVMFKSDKFL 530 IIPLHSLMPT 540 VNQTQVFKRT 550 PPGVRKIVIA 560 TNIAETSITI 570 DDVVYVIDGG 580 KIKETHFDTQ 590 NNISTMSAEW 600 VSKANAKQRK 610 GRAGRVQPGH 620 CYHLYNGLRA 630 SLLDDYQLPE 640 ILRTPLEELC 650 LQIKILRLGG 660 IAYFLSRLMD 670 PPSNEAVLLS 680 IRHLMELNAL 690 DKQEELTPLG 700 VHLARLPVEP 710 HIGKMILFGA 720 LFCCLDPVLT 730 IAASLSFKDP 740 FVIPLGKEKI 750 ADARRKELAK 760 DTRSDHLTVV 770 NAFEGWEEAR 780 RRGFRYEKDY 790 CWEYFLSSNT 800 LQMLHNMKGQ 810 FAEHLLGAGF 820 VSSRNPKDPE 830 SNINSDNEKI 840 IKAVICAGLY 850 PKVAKIRLNL 860 GKKRKMVKVY 870 TKTDGLVAVH 880 PKSVNVEQTD 890 FHYNWLIYHL 900 KMRTSSIYLY 910 DCTEVSPYCL 920 LFFGGDISIQ 930 KDNDQETIAV 940 DEWIVFQSPA 950 RIAHLVKELR 960 KELDILLQEK 970 IESPHPVDWN 980 DTKSRDCAVL 990 SAIIDLIKTQ 1000 EKATPRNFPP RFQDGYYS

Gene Ontology

Classification	GO ID	Description
Biological Process	GO:0043123	positive regulation of canonical NF-kappaB signal transduction
Cellular Component	GO:0030424	axon
Cellular Component	GO:0000781	chromosome, telomeric region
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0010494	cytoplasmic stress granule
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0030425	dendrite
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0043204	perikaryon
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0008094	ATP-dependent activity, acting on DNA
Molecular Function	GO:0003678	DNA helicase activity
Molecular Function	GO:0003725	double-stranded RNA binding
Molecular Function	GO:0051880	G-quadruplex DNA binding
Molecular Function	GO:0002151	G-quadruplex RNA binding
Molecular Function	GO:0160225	G-quadruplex unwinding activity
Molecular Function	GO:0042826	histone deacetylase binding
Molecular Function	GO:0000287	magnesium ion binding
Molecular Function	GO:0035925	mRNA 3'-UTR AU-rich region binding
Molecular Function	GO:0003730	mRNA 3'-UTR binding
Molecular Function	GO:0048027	mRNA 5'-UTR binding
Molecular Function	GO:0070883	pre-miRNA binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003724	RNA helicase activity
Molecular Function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function	GO:0003697	single-stranded DNA binding
Molecular Function	GO:0070034	telomerase RNA binding
Molecular Function	GO:0000976	transcription cis-regulatory region binding
Biological Process	GO:0061158	3'-UTR-mediated mRNA destabilization
Biological Process	GO:0030154	cell differentiation
Biological Process	GO:1903843	cellular response to arsenite ion
Biological Process	GO:0034605	cellular response to heat
Biological Process	GO:0034644	cellular response to UV
Biological Process	GO:0051607	defense response to virus
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0045087	innate immune response
Biological Process	GO:0017148	negative regulation of translation
Biological Process	GO:0001503	ossification
Biological Process	GO:0051891	positive regulation of cardioblast differentiation
Biological Process	GO:2000767	positive regulation of cytoplasmic translation
Biological Process	GO:0061003	positive regulation of dendritic spine morphogenesis
Biological Process	GO:0010628	positive regulation of gene expression
Biological Process	GO:1901534	positive regulation of hematopoietic progenitor cell differentiation
Biological Process	GO:0032727	positive regulation of interferon-alpha production
Biological Process	GO:1904582	positive regulation of intracellular mRNA localization
Biological Process	GO:0031442	positive regulation of mRNA 3'-end processing
Biological Process	GO:0002735	positive regulation of myeloid dendritic cell cytokine production
Biological Process	GO:1900153	positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Biological Process	GO:0032206	positive regulation of telomere maintenance
Biological Process	GO:1904358	positive regulation of telomere maintenance via telomere lengthening
Biological Process	GO:0045944	positive regulation of transcription by RNA polymerase II
Biological Process	GO:0060261	positive regulation of transcription initiation by RNA polymerase II
Biological Process	GO:0045995	regulation of embryonic development
Biological Process	GO:0043488	regulation of mRNA stability
Biological Process	GO:0006359	regulation of transcription by RNA polymerase III
Biological Process	GO:0007283	spermatogenesis
Biological Process	GO:0090669	telomerase RNA stabilization

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.