Search Results

Overview

Uniprot IDQ9H6R3
Protein NameAcyl-CoA synthetase short-chain family member 3, mitochondrial
Gene NameACSS3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
128 IENGKGDKIAIIYDS
162 KLAGVLVKHGIKKGD
205 IFGGFASKELSSRID
411 DPGAALGKQYSLTRF
493 ILDDNMQKLKARCLG
524 WKNQEAFKHLYFEKF
594 VGKEDPLKGHVPLAL
662 LSAIVNGKPYKITST
79 DPERFWGKAAEQISW
92 SWYKPWTKTLENKHS
97 WTKTLENKHSPSTRW

Function

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:28003429). Propionate is the preferred substrate (PubMed:28003429). Can utilize acetate and butyrate with a much lower affinity (By similarity)

Protein Sequence

10 MKPSWLQCRK 20 VTSAGGLGGP 30 LPGSSPARGA 40 GAALRALVVP 50 GPRGGLGGRG 60 CRALSSGSGS 70 EYKTHFAASV 80 TDPERFWGKA 90 AEQISWYKPW 100 TKTLENKHSP 110 STRWFVEGML 120 NICYNAVDRH 130 IENGKGDKIA 140 IIYDSPVTNT 150 KATFTYKEVL 160 EQVSKLAGVL 170 VKHGIKKGDT 180 VVIYMPMIPQ 190 AMYTMLACAR 200 IGAIHSLIFG 210 GFASKELSSR 220 IDHVKPKVVV 230 TASFGIEPGR 240 RVEYVPLVEE 250 ALKIGQHKPD 260 KILIYNRPNM 270 EAVPLAPGRD 280 LDWDEEMAKA 290 QSHDCVPVLS 300 EHPLYILYTS 310 GTTGLPKGVI 320 RPTGGYAVML 330 HWSMSSIYGL 340 QPGEVWWAAS 350 DLGWVVGHSY 360 ICYGPLLHGN 370 TTVLYEGKPV 380 GTPDAGAYFR 390 VLAEHGVAAL 400 FTAPTAIRAI 410 RQQDPGAALG 420 KQYSLTRFKT 430 LFVAGERCDV 440 ETLEWSKNVF 450 RVPVLDHWWQ 460 TETGSPITAS 470 CVGLGNSKTP 480 PPGQAGKSVP 490 GYNVMILDDN 500 MQKLKARCLG 510 NIVVKLPLPP 520 GAFSGLWKNQ 530 EAFKHLYFEK 540 FPGYYDTMDA 550 GYMDEEGYLY 560 VMSRVDDVIN 570 VAGHRISAGA 580 IEESILSHGT 590 VADCAVVGKE 600 DPLKGHVPLA 610 LCVLRKDINA 620 TEEQVLEEIV 630 KHVRQNIGPV 640 AAFRNAVFVK 650 QLPKTRSGKI 660 PRSALSAIVN 670 GKPYKITSTI 680 EDPSIFGHVE EMLKQA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0003987 acetate-CoA ligase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0031956 medium-chain fatty acid-CoA ligase activity
Molecular Function GO:0050218 propionate-CoA ligase activity
Biological Process GO:0046951 ketone body biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.