Search Results

Overview

Uniprot ID	Q9H8Y5
Protein Name	tRNA endonuclease ANKZF1
Gene Name	ANKZF1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
310	RSLFFGGKGAPLQRG

Function

Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation (PubMed:29632312, PubMed:30244831, PubMed:31011209). The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts (PubMed:29632312, PubMed:30244831, PubMed:31011209). ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs (PubMed:31011209). ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1 (PubMed:31011209, PubMed:32075755). Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (PubMed:28302725)

Protein Sequence

10 MSPAPDAAPA 20 PASISLFDLS 30 ADAPVFQGLS 40 LVSHAPGEAL 50 ARAPRTSCSG 60 SGERESPERK 70 LLQGPMDISE 80 KLFCSTCDQT 90 FQNHQEQREH 100 YKLDWHRFNL 110 KQRLKDKPLL 120 SALDFEKQSS 130 TGDLSSISGS 140 EDSDSASEED 150 LQTLDRERAT 160 FEKLSRPPGF 170 YPHRVLFQNA 180 QGQFLYAYRC 190 VLGPHQDPPE 200 EAELLLQNLQ 210 SRGPRDCVVL 220 MAAAGHFAGA 230 IFQGREVVTH 240 KTFHRYTVRA 250 KRGTAQGLRD 260 ARGGPSHSAG 270 ANLRRYNEAT 280 LYKDVRDLLA 290 GPSWAKALEE 300 AGTILLRAPR 310 SGRSLFFGGK 320 GAPLQRGDPR 330 LWDIPLATRR 340 PTFQELQRVL 350 HKLTTLHVYE 360 EDPREAVRLH 370 SPQTHWKTVR 380 EERKKPTEEE 390 IRKICRDEKE 400 ALGQNEESPK 410 QGSGSEGEDG 420 FQVELELVEL 430 TVGTLDLCES 440 EVLPKRRRRK 450 RNKKEKSRDQ 460 EAGAHRTLLQ 470 QTQEEEPSTQ 480 SSQAVAAPLG 490 PLLDEAKAPG 500 QPELWNALLA 510 ACRAGDVGVL 520 KLQLAPSPAD 530 PRVLSLLSAP 540 LGSGGFTLLH 550 AAAAAGRGSV 560 VRLLLEAGAD 570 PTVQDSRARP 580 PYTVAADKST 590 RNEFRRFMEK 600 NPDAYDYNKA 610 QVPGPLTPEM 620 EARQATRKRE 630 QKAARRQREE 640 QQQRQQEQEE 650 REREEQRRFA 660 ALSDREKRAL 670 AAERRLAAQL 680 GAPTSPIPDS 690 AIVNTRRCWS 700 CGASLQGLTP 710 FHYLDFSFCS 720 TRCLQDHRRQ AGRPSS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016020	membrane
Molecular Function	GO:0140101	catalytic activity, acting on a tRNA
Molecular Function	GO:0016787	hydrolase activity
Molecular Function	GO:0004521	RNA endonuclease activity
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0070301	cellular response to hydrogen peroxide
Biological Process	GO:0036503	ERAD pathway
Biological Process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
Biological Process	GO:0072344	rescue of stalled cytosolic ribosome

Reference

[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.