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Overview

Uniprot IDQ9HAN9
Protein NameNicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
Gene NameNMNAT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
128 ERPGRKRKWTETQDS
138 ETQDSSQKKSLEPKT
139 TQDSSQKKSLEPKTK
57 PVGDAYKKKGLIPAY

Function

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults (By similarity). Neural protection does not correlate with cellular NAD(+) levels but may still require enzyme activity (By similarity)

Protein Sequence

10 MENSEKTEVV 20 LLACGSFNPI 30 TNMHLRLFEL 40 AKDYMNGTGR 50 YTVVKGIISP 60 VGDAYKKKGL 70 IPAYHRVIMA 80 ELATKNSKWV 90 EVDTWESLQK 100 EWKETLKVLR 110 HHQEKLEASD 120 CDHQQNSPTL 130 ERPGRKRKWT 140 ETQDSSQKKS 150 LEPKTKAVPK 160 VKLLCGADLL 170 ESFAVPNLWK 180 SEDITQIVAN 190 YGLICVTRAG 200 NDAQKFIYES 210 DVLWKHRSNI 220 HVVNEWIAND 230 ISSTKIRRAL 240 RRGQSIRYLV 250 PDLVQEYIEK 260 HNLYSSESED 270 RNAGVILAPL QRNTAEAKT

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0000309 nicotinamide-nucleotide adenylyltransferase activity
Molecular Function GO:0004515 nicotinate-nucleotide adenylyltransferase activity
Molecular Function GO:0140768 protein ADP-ribosyltransferase-substrate adaptor activity
Biological Process GO:1990966 ATP generation from poly-ADP-D-ribose
Biological Process GO:0034355 NAD+ biosynthetic process via the salvage pathway
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0009165 nucleotide biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.