Search Results

Overview

Uniprot IDQ9HAW4
Protein NameClaspin
Gene NameCLSPN
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
105 TKIKRIYKTVADSDE
1265 QPKAVLQKLAALSDH
319 KTIHDFFKRKPRPTC
537 ALKQRFWKHANPAAK
824 SLVSSASKSSGKLSE

Function

Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation (PubMed:12766152, PubMed:15190204, PubMed:15707391, PubMed:16123041). Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins (PubMed:12766152, PubMed:15096610, PubMed:15707391, PubMed:16123041). Also required to maintain normal rates of replication fork progression during unperturbed DNA replication. Binds directly to DNA, with particular affinity for branched or forked molecules and interacts with multiple protein components of the replisome such as the MCM2-7 complex and TIMELESS (PubMed:15226314, PubMed:34694004, PubMed:35585232). Important for initiation of DNA replication, recruits kinase CDC7 to phosphorylate MCM2-7 components (PubMed:27401717)

Protein Sequence

10 MTGEVGSEVH 20 LEINDPNVIS 30 QEEADSPSDS 40 GQGSYETIGP 50 LSEGDSDEEI 60 FVSKKLKNRK 70 VLQDSDSETE 80 DTNASPEKTT 90 YDSAEEENKE 100 NLYAGKNTKI 110 KRIYKTVADS 120 DESYMEKSLY 130 QENLEAQVKP 140 CLELSLQSGN 150 STDFTTDRKS 160 SKKHIHDKEG 170 TAGKAKVKSK 180 RRLEKEERKM 190 EKIRQLKKKE 200 TKNQEDDVEQ 210 PFNDSGCLLV 220 DKDLFETGLE 230 DENNSPLEDE 240 ESLESIRAAV 250 KNKVKKHKKK 260 EPSLESGVHS 270 FEEGSELSKG 280 TTRKERKAAR 290 LSKEALKQLH 300 SETQRLIRES 310 ALNLPYHMPE 320 NKTIHDFFKR 330 KPRPTCHGNA 340 MALLKSSKYQ 350 SSHHKEIIDT 360 ANTTEMNSDH 370 HSKGSEQTTG 380 AENEVETNAL 390 PVVSKETQII 400 TGSDESCRKD 410 LVKNEELEIQ 420 EKQKQSDIRP 430 SPGDSSVLQQ 440 ESNFLGNNHS 450 EECQVGGLVA 460 FEPHALEGEG 470 PQNPEETDEK 480 VEEPEQQNKS 490 SAVGPPEKVR 500 RFTLDRLKQL 510 GVDVSIKPRL 520 GADEDSFVIL 530 EPETNRELEA 540 LKQRFWKHAN 550 PAAKPRAGQT 560 VNVNVIVKDM 570 GTDGKEELKA 580 DVVPVTLAPK 590 KLDGASHTKP 600 GEKLQVLKAK 610 LQEAMKLRRF 620 EERQKRQALF 630 KLDNEDGFEE 640 EEEEEEEMTD 650 ESEEDGEEKV 660 EKEEKEEELE 670 EEEEKEEEEE 680 EEGNQETAEF 690 LLSSEEIETK 700 DEKEMDKENN 710 DGSSEIGKAV 720 GFLSVPKSLS 730 SDSTLLLFKD 740 SSSKMGYFPT 750 EEKSETDENS 760 GKQPSKLDED 770 DSCSLLTKES 780 SHNSSFELIG 790 STIPSYQPCN 800 RQTGRGTSFF 810 PTAGGFRSPS 820 PGLFRASLVS 830 SASKSSGKLS 840 EPSLPIEDSQ 850 DLYNASPEPK 860 TLFLGAGDFQ 870 FCLEDDTQSQ 880 LLDADGFLNV 890 RNHRNQYQAL 900 KPRLPLASMD 910 ENAMDANMDE 920 LLDLCTGKFT 930 SQAEKHLPRK 940 SDKKENMEEL 950 LNLCSGKFTS 960 QDASTPASSE 970 LNKQEKESSM 980 GDPMEEALAL 990 CSGSFPTDKE 1000 EEDEEEEFGD 1010 FRLVSNDNEF 1020 DSDEDEHSDS 1030 GNDLALEDHE 1040 DDDEEELLKR 1050 SEKLKRQMRL 1060 RKYLEDEAEV 1070 SGSDVGSEDE 1080 YDGEEIDEYE 1090 EDVIDEVLPS 1100 DEELQSQIKK 1110 IHMKTMLDDD 1120 KRQLRLYQER 1130 YLADGDLHSD 1140 GPGRMRKFRW 1150 KNIDDASQMD 1160 LFHRDSDDDQ 1170 TEEQLDESEA 1180 RWRKERIERE 1190 QWLRDMAQQG 1200 KITAEEEEEI 1210 GEDSQFMILA 1220 KKVTAKALQK 1230 NASRPMVIQE 1240 SKSLLRNPFE 1250 AIRPGSAQQV 1260 KTGSLLNQPK 1270 AVLQKLAALS 1280 DHNPSAPRNS 1290 RNFVFHTLSP 1300 VKAEAAKESS 1310 KSQVKKRGPS 1320 FMTSPSPKHL 1330 KTDDSTSGLT RSIFKYLES

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0010997 anaphase-promoting complex binding
Molecular Function GO:0000217 DNA secondary structure binding
Biological Process GO:0000077 DNA damage checkpoint signaling
Biological Process GO:0006281 DNA repair
Biological Process GO:0000076 DNA replication checkpoint signaling
Biological Process GO:0033314 mitotic DNA replication checkpoint signaling
Biological Process GO:0007095 mitotic G2 DNA damage checkpoint signaling

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.