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Overview

Uniprot IDQ9HAZ2
Protein NameHistone-lysine N-methyltransferase PRDM16
Gene NamePRDM16
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
316 YKCDQCPKAFNWKSN

Function

Transcription regulator that acts both as a histone methyltransferase or chromatin adapter, depending on the context (PubMed:12816872). In the cytoplasm, acts as a histone methyltransferase, which catalyzes monomethylation of 'Lys-9' of free histone H3 (H3K9me1) during translation (By similarity). Monomethylated histone H3 is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases (SUV39H1 and SUV39H2) use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (H3K9me3) (By similarity). Probably one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). In the nucleus, acts as a key chromatin adapter that mediates differentiation of brown and beige adipocytes, which are specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding (By similarity). Following recruitment to chromatin by PPARG nuclear receptor, promotes differentiation of myoblastic precursors into brown adipose cells (By similarity). Mechanistically, acts by mediating recruitment of (1) EHMT1 histone methyltransferase, thereby inhibiting the expression of white adipose-selective genes or (2) mediator complex, activating genes that are highly expressed in brown adipocytes, such as UCP1, PPARA, and PPARGC1A (By similarity). Also mediates differentiation of beige adipocytes from white adipose cells following recruitment by PPARG (By similarity). In addition to adipocyte differentiation, also involved in tolerance to gut microbiota: following recruitment to chromatin by RORgammaT (RORC) in a subset of antigen-presenting cells, promotes differentiation of peripherally-induced regulatory T-cells (pTreg), which suppress inflammatory responses to commensal microorganisms (By similarity). Also required in adult heart to preserve mitochondrial function and inhibit hypertrophy with advanced age (By similarity). Functions as a repressor of TGF-beta signaling (PubMed:19049980)

Protein Sequence

10 MRSKARARKL 20 AKSDGDVVNN 30 MYEPNRDLLA 40 SHSAEDEAED 50 SAMSPIPVGP 60 PSPFPTSEDF 70 TPKEGSPYEA 80 PVYIPEDIPI 90 PADFELRESS 100 IPGAGLGVWA 110 KRKMEAGERL 120 GPCVVVPRAA 130 AKETDFGWEQ 140 ILTDVEVSPQ 150 EGCITKISED 160 LGSEKFCVDA 170 NQAGAGSWLK 180 YIRVACSCDD 190 QNLTMCQISE 200 QIYYKVIKDI 210 EPGEELLVHV 220 KEGVYPLGTV 230 PPGLDEEPTF 240 RCDECDELFQ 250 SKLDLRRHKK 260 YTCGSVGAAL 270 YEGLAEELKP 280 EGLGGGSGQA 290 HECKDCERMF 300 PNKYSLEQHM 310 VIHTEEREYK 320 CDQCPKAFNW 330 KSNLIRHQMS 340 HDSGKRFECE 350 NCVKVFTDPS 360 NLQRHIRSQH 370 VGARAHACPD 380 CGKTFATSSG 390 LKQHKHIHST 400 VKPFICEVCH 410 KSYTQFSNLC 420 RHKRMHADCR 430 TQIKCKDCGQ 440 MFSTTSSLNK 450 HRRFCEGKNH 460 YTPGGIFAPG 470 LPLTPSPMMD 480 KAKPSPSLNH 490 ASLGFNEYFP 500 SRPHPGSLPF 510 STAPPTFPAL 520 TPGFPGIFPP 530 SLYPRPPLLP 540 PTSLLKSPLN 550 HTQDAKLPSP 560 LGNPALPLVS 570 AVSNSSQGTT 580 AAAGPEEKFE 590 SRLEDSCVEK 600 LKTRSSDMSD 610 GSDFEDVNTT 620 TGTDLDTTTG 630 TGSDLDSDVD 640 SDPDKDKGKG 650 KSAEGQPKFG 660 GGLAPPGAPN 670 SVAEVPVFYS 680 QHSFFPPPDE 690 QLLTATGAAG 700 DSIKAIASIA 710 EKYFGPGFMG 720 MQEKKLGSLP 730 YHSAFPFQFL 740 PNFPHSLYPF 750 TDRALAHNLL 760 VKAEPKSPRD 770 ALKVGGPSAE 780 CPFDLTTKPK 790 DVKPILPMPK 800 GPSAPASGEE 810 QPLDLSIGSR 820 ARASQNGGGR 830 EPRKNHVYGE 840 RKLGAGEGLP 850 QVCPARMPQQ 860 PPLHYAKPSP 870 FFMDPIYSRV 880 EKRKVTDPVG 890 ALKEKYLRPS 900 PLLFHPQMSA 910 IETMTEKLES 920 FAAMKADSGS 930 SLQPLPHHPF 940 NFRSPPPTLS 950 DPILRKGKER 960 YTCRYCGKIF 970 PRSANLTRHL 980 RTHTGEQPYR 990 CKYCDRSFSI 1000 SSNLQRHVRN 1010 IHNKEKPFKC 1020 HLCNRCFGQQ 1030 TNLDRHLKKH 1040 EHENAPVSQH 1050 PGVLTNHLGT 1060 SASSPTSESD 1070 NHALLDEKED 1080 SYFSEIRNFI 1090 ANSEMNQAST 1100 RTEKRADMQI 1110 VDGSAQCPGL 1120 ASEKQEDVEE 1130 EDDDDLEEDD 1140 EDSLAGKSQD 1150 DTVSPAPEPQ 1160 AAYEDEEDEE 1170 PAASLAVGFD 1180 HTRRCAEDHE 1190 GGLLALEPMP 1200 TFGKGLDLRR 1210 AAEEAFEVKD 1220 VLNSTLDSEA 1230 LKHTLCRQAK 1240 NQAYAMMLSL 1250 SEDTPLHTPS 1260 QGSLDAWLKV 1270 TGATSESGAF HPINHL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0017053 transcription repressor complex
Molecular Function GO:0140463 chromatin-protein adaptor activity
Molecular Function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
Molecular Function GO:0140297 DNA-binding transcription factor binding
Molecular Function GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular Function GO:0140938 histone H3 methyltransferase activity
Molecular Function GO:0046974 histone H3K9 methyltransferase activity
Molecular Function GO:0140948 histone H3K9 monomethyltransferase activity
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:0000976 transcription cis-regulatory region binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0003712 transcription coregulator activity
Molecular Function GO:0003714 transcription corepressor activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0160274 beige fat cell differentiation
Biological Process GO:0050873 brown fat cell differentiation
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0070828 heterochromatin organization
Biological Process GO:0032259 methylation
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0030853 negative regulation of granulocyte differentiation
Biological Process GO:0051148 negative regulation of muscle cell differentiation
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
Biological Process GO:0160275 negative regulation of white fat cell differentiation
Biological Process GO:0120162 positive regulation of cold-induced thermogenesis
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0071168 protein localization to chromatin
Biological Process GO:0051604 protein maturation
Biological Process GO:0043457 regulation of cellular respiration
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0045066 regulatory T cell differentiation
Biological Process GO:0002394 tolerance induction in gut-associated lymphoid tissue

Reference

PMID: N/A