Search Results
Overview
| Uniprot ID | Q9HCE5 |
|---|---|
| Protein Name | N(6)-adenosine-methyltransferase non-catalytic subunit METTL14 |
| Gene Name | METTL14 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 63 | DTSAPNAKRKYLDEG |
| 65 | SAPNAKRKYLDEGET |
Function
The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27281194, PubMed:27373337, PubMed:29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27281194, PubMed:27373337, PubMed:27627798, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Molecular Function | GO:0003729 | mRNA binding |
| Molecular Function | GO:0001734 | mRNA m(6)A methyltransferase activity |
| Biological Process | GO:0006974 | DNA damage response |
| Biological Process | GO:0021861 | forebrain radial glial cell differentiation |
| Biological Process | GO:0042063 | gliogenesis |
| Biological Process | GO:0061157 | mRNA destabilization |
| Biological Process | GO:0016556 | mRNA modification |
| Biological Process | GO:0006397 | mRNA processing |
| Biological Process | GO:0000398 | mRNA splicing, via spliceosome |
| Biological Process | GO:0048255 | mRNA stabilization |
| Biological Process | GO:1901533 | negative regulation of hematopoietic progenitor cell differentiation |
| Biological Process | GO:0045727 | positive regulation of translation |
| Biological Process | GO:0031053 | primary miRNA processing |
| Biological Process | GO:0045664 | regulation of neuron differentiation |
| Biological Process | GO:0045580 | regulation of T cell differentiation |
| Biological Process | GO:0031667 | response to nutrient levels |
| Biological Process | GO:0001510 | RNA methylation |
| Biological Process | GO:0007283 | spermatogenesis |
| Biological Process | GO:0019827 | stem cell population maintenance |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.
[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.