Search Results

Overview

Uniprot IDQ9HCK8
Protein NameATP-dependent chromatin remodeler CHD8
Gene NameCHD8
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
2241 TAAAQFTKLRRGMDE
2286 GHPLFHKKKGNRKKL
2416 PIAPESSKKRARRMR
2417 IAPESSKKRARRMRP
532 SKTKGKSKLNTITPV
542 TITPVVGKKRKRNTS
543 ITPVVGKKRKRNTSS
573 DEESSIQKRRSNRQV
965 CKLLDSLKHMDLEHK

Function

ATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP (PubMed:28533432). Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Regulates alternative splicing of a core group of genes involved in neuronal differentiation, cell cycle and DNA repair. Enables H3K36me3-coupled transcription elongation and co-transcriptional RNA processing likely via interaction with HNRNPL

Protein Sequence

10 MADPIMDLFD 20 DPNLFGLDSL 30 TDDSFNQVTQ 40 DPIEEALGLP 50 SSLDSLDQMN 60 QDGGGGDVGN 70 SSASELVPPP 80 EETAPTELSK 90 ESTAPAPESI 100 TLHDYTTQPA 110 SQEQPAQPVL 120 QTSTPTSGLL 130 QVSKSQEILS 140 QGNPFMGVSA 150 TAVSSSSAGG 160 QPPQSAPKIV 170 ILKAPPSSSV 180 TGAHVAQIQA 190 QGITSTAQPL 200 VAGTANGGKV 210 TFTKVLTGTP 220 LRPGVSIVSG 230 NTVLAAKVPG 240 NQAAVQRIVQ 250 PSRPVKQLVL 260 QPVKGSAPAG 270 NPGATGPPLK 280 PAVTLTSTPT 290 QGESKRITLV 300 LQQPQSGGPQ 310 GHRHVVLGSL 320 PGKIVLQGNQ 330 LAALTQAKNA 340 QGQPAKVVTI 350 QLQVQQPQQK 360 IQIVPQPPSS 370 QPQPQQPPST 380 QPVTLSSVQQ 390 AQIMGPGQSP 400 GQRLSVPVKV 410 VLQPQAGSSQ 420 GASSGLSVVK 430 VLSASEVAAL 440 SSPASSAPHS 450 GGKTGMEENR 460 RLEHQKKQEK 470 ANRIVAEAIA 480 RARARGEQNI 490 PRVLNEDELP 500 SVRPEEEGEK 510 KRRKKSAGER 520 LKEEKPKKSK 530 TSGASKTKGK 540 SKLNTITPVV 550 GKKRKRNTSS 560 DNSDVEVMPA 570 QSPREDEESS 580 IQKRRSNRQV 590 KRKKYTEDLD 600 IKITDDEEEE 610 EVDVTGPIKP 620 EPILPEPVQE 630 PDGETLPSMQ 640 FFVENPSEED 650 AAIVDKVLSM 660 RIVKKELPSG 670 QYTEAEEFFV 680 KYKNYSYLHC 690 EWATISQLEK 700 DKRIHQKLKR 710 FKTKMAQMRH 720 FFHEDEEPFN 730 PDYVEVDRIL 740 DESHSIDKDN 750 GEPVIYYLVK 760 WCSLPYEDST 770 WELKEDVDEG 780 KIREFKRIQS 790 RHPELKRVNR 800 PQASAWKKLE 810 LSHEYKNRNQ 820 LREYQLEGVN 830 WLLFNWYNRQ 840 NCILADEMGL 850 GKTIQSIAFL 860 QEVYNVGIHG 870 PFLVIAPLST 880 ITNWEREFNT 890 WTEMNTIVYH 900 GSLASRQMIQ 910 QYEMYCKDSR 920 GRLIPGAYKF 930 DALITTFEMI 940 LSDCPELREI 950 EWRCVIIDEA 960 HRLKNRNCKL 970 LDSLKHMDLE 980 HKVLLTGTPL 990 QNTVEELFSL 1000 LHFLEPSQFP 1010 SESEFLKDFG 1020 DLKTEEQVQK 1030 LQAILKPMML 1040 RRLKEDVEKN 1050 LAPKQETIIE 1060 VELTNIQKKY 1070 YRAILEKNFS 1080 FLSKGAGHTN 1090 MPNLLNTMME 1100 LRKCCNHPYL 1110 INGAEEKILT 1120 EFREACHIIP 1130 HDFHLQAMVR 1140 SAGKLVLIDK 1150 LLPKLKAGGH 1160 KVLIFSQMVR 1170 CLDILEDYLI 1180 QRRYLYERID 1190 GRVRGNLRQA 1200 AIDRFSKPDS 1210 DRFVFLLCTR 1220 AGGLGINLTA 1230 ADTCIIFDSD 1240 WNPQNDLQAQ 1250 ARCHRIGQSK 1260 AVKVYRLITR 1270 NSYEREMFDK 1280 ASLKLGLDKA 1290 VLQSMSGRDG 1300 NITGIQQFSK 1310 KEIEDLLRKG 1320 AYAAIMEEDD 1330 EGSKFCEEDI 1340 DQILLRRTTT 1350 ITIESEGKGS 1360 TFAKASFVAS 1370 ENRTDISLDD 1380 PNFWQKWAKK 1390 ADLDMDLLNS 1400 KNNLVIDTPR 1410 VRKQTRHFST 1420 LKDDDLVEFS 1430 DLESEDDERP 1440 RSRRHDRHHA 1450 YGRTDCFRVE 1460 KHLLVYGWGR 1470 WRDILSHGRF 1480 KRRMTERDVE 1490 TICRAILVYC 1500 LLHYRGDENI 1510 KGFIWDLISP 1520 AENGKTKELQ 1530 NHSGLSIPVP 1540 RGRKGKKVKS 1550 QSTFDIHKAD 1560 WIRKYNPDTL 1570 FQDESYKKHL 1580 KHQCNKVLLR 1590 VRMLYYLRQE 1600 VIGDQAEKVL 1610 GGAIASEIDI 1620 WFPVVDQLEV 1630 PTTWWDSEAD 1640 KSLLIGVFKH 1650 GYEKYNTMRA 1660 DPALCFLEKA 1670 GRPDDKAIAA 1680 EHRVLDNFSD 1690 IVEGVDFDKD 1700 CEDPEYKPLQ 1710 GPPKDQDDEG 1720 DPLMMMDEEI 1730 SVIDGDEAQV 1740 TQQPGHLFWP 1750 PGSALTARLR 1760 RLVTAYQRSY 1770 KREQMKIEAA 1780 ERGDRRRRRC 1790 EAAFKLKEIA 1800 RREKQQRWTR 1810 REQTDFYRVV 1820 STFGVEYDPD 1830 TMQFHWDRFR 1840 TFARLDKKTD 1850 ESLTKYFHGF 1860 VAMCRQVCRL 1870 PPAAGDEPPD 1880 PNLFIEPITE 1890 ERASRTLYRI 1900 ELLRRLREQV 1910 LCHPLLEDRL 1920 ALCQPPGPEL 1930 PKWWEPVRHD 1940 GELLRGAARH 1950 GVSQTDCNIM 1960 QDPDFSFLAA 1970 RMNYMQNHQA 1980 GAPAPSLSRC 1990 STPLLHQQYT 2000 SRTASPLPLR 2010 PDAPVEKSPE 2020 ETATQVPSLE 2030 SLTLKLEHEV 2040 VARSRPTPQD 2050 YEMRVSPSDT 2060 TPLVSRSVPP 2070 VKLEDEDDSD 2080 SELDLSKLSP 2090 SSSSSSSSSS 2100 SSSSTDESED 2110 EKEEKLTDQS 2120 RSKLYDEESL 2130 LSLTMSQDGF 2140 PNEDGEQMTP 2150 ELLLLQERQR 2160 ASEWPKDRVL 2170 INRIDLVCQA 2180 VLSGKWPSSR 2190 RSQEMVTGGI 2200 LGPGNHLLDS 2210 PSLTPGEYGD 2220 SPVPTPRSSS 2230 AASMAEEEAS 2240 AVSTAAAQFT 2250 KLRRGMDEKE 2260 FTVQIKDEEG 2270 LKLTFQKHKL 2280 MANGVMGDGH 2290 PLFHKKKGNR 2300 KKLVELEVEC 2310 MEEPNHLDVD 2320 LETRIPVINK 2330 VDGTLLVGED 2340 APRRAELEMW 2350 LQGHPEFAVD 2360 PRFLAYMEDR 2370 RKQKWQRCKK 2380 NNKAELNCLG 2390 MEPVQTANSR 2400 NGKKGHHTET 2410 VFNRVLPGPI 2420 APESSKKRAR 2430 RMRPDLSKMM 2440 ALMQGGSTGS 2450 LSLHNTFQHS 2460 SSGLQSVSSL 2470 GHSSATSASL 2480 PFMPFVMGGA 2490 PSSPHVDSST 2500 MLHHHHHHPH 2510 PHHHHHHHPG 2520 LRAPGYPSSP 2530 VTTASGTTLR 2540 LPPLQPEEDD 2550 DEDEEDDDDL 2560 SQGYDSSERD 2570 FSLIDDPMMP 2580 ANSDSSEDAD D

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0071339 MLL1 complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0140658 ATP-dependent chromatin remodeler activity
Molecular Function GO:0008013 beta-catenin binding
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0042393 histone binding
Molecular Function GO:0140002 histone H3K4me3 reader activity
Molecular Function GO:0002039 p53 binding
Biological Process GO:0007420 brain development
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0048565 digestive tract development
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0006397 mRNA processing
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0090090 negative regulation of canonical Wnt signaling pathway
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0045945 positive regulation of transcription by RNA polymerase III
Biological Process GO:0016055 Wnt signaling pathway

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.