Search Results

Overview

Uniprot ID	Q9NRN9
Protein Name	rRNA N(6)-adenosine-methyltransferase METTL5
Gene Name	METTL5
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
191	YDLPASYKFHKKKSV

Function

Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA (PubMed:31328227, PubMed:32217665, PubMed:33357433, PubMed:33428944, PubMed:35033535). N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation (PubMed:33357433)

Protein Sequence

10 MKKVRLKELE 20 SRLQQVDGFE 30 KPKLLLEQYP 40 TRPHIAACML 50 YTIHNTYDDI 60 ENKVVADLGC 70 GCGVLSIGTA 80 MLGAGLCVGF 90 DIDEDALEIF 100 NRNAEEFELT 110 NIDMVQCDVC 120 LLSNRMSKSF 130 DTVIMNPPFG 140 TKNNKGTDMA 150 FLKTALEMAR 160 TAVYSLHKSS 170 TREHVQKKAA 180 EWKIKIDIIA 190 ELRYDLPASY 200 KFHKKKSVDI EVDLIRFSF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0001650	fibrillar center
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0098794	postsynapse
Cellular Component	GO:0098793	presynapse
Molecular Function	GO:0003676	nucleic acid binding
Molecular Function	GO:0008988	rRNA (adenine-N6-)-methyltransferase activity
Molecular Function	GO:1904047	S-adenosyl-L-methionine binding
Biological Process	GO:0045727	positive regulation of translation
Biological Process	GO:0031167	rRNA methylation
Biological Process	GO:0006364	rRNA processing
Biological Process	GO:0048863	stem cell differentiation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.